Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Colloids Surf B Biointerfaces. 2020 Oct;194:111205. doi: 10.1016/j.colsurfb.2020.111205. Epub 2020 Jun 17.
The effects of protein adsorption on the polymer brush surfaces with well-defined chemical structures and physical properties were examined with respect to initial protein adsorption, structural changes to the adsorbed proteins, and subsequent cell adhesion. Four polymer brush surfaces with different hydrophilicities and charge states were prepared. The molecular interaction forces during adsorption-desorption processes of protein on the polymer brush surfaces depending on the chemical structure of the polymer were determined. Crucially, these molecular interactions affected the adsorption behavior and structural changes of fibronectin (FN), a cell-adhesive protein, used in this study. Adsorption of FN onto the zwitterionic polymer and anionic polymer surfaces was difficult, however significant protein adsorption to the hydrophobic and cationic surfaces was observed. Further, the structural changes to the adhered FN on these surfaces were significant. Subsequent cell adhesion experiments revealed that the adhered cell density was correlated with the amount of adsorbed FN and the degree of FN structural change. In addition, the cationic surface inhibited cell proliferation behavior. These results indicate that cellular responses can be indirectly regulated by controlling the molecular interactions which induced the structural change of adsorbed proteins via the material surface properties.
本研究考察了具有明确化学结构和物理性质的聚合物刷表面上蛋白质吸附的影响，包括初始蛋白质吸附、吸附蛋白质的结构变化和随后的细胞黏附。制备了四种具有不同亲水性和电荷状态的聚合物刷表面。确定了蛋白质在聚合物刷表面上吸附-解吸过程中的分子相互作用力取决于聚合物的化学结构。至关重要的是，这些分子相互作用影响了本研究中使用的细胞黏附蛋白纤维连接蛋白 (FN) 的吸附行为和结构变化。FN 不易吸附到两性离子聚合物和阴离子聚合物表面上，但在疏水性和阳离子表面上观察到显著的蛋白质吸附。此外，黏附在这些表面上的 FN 的结构变化也很明显。随后的细胞黏附实验表明，黏附细胞的密度与吸附的 FN 量和 FN 结构变化程度相关。此外，阳离子表面抑制了细胞增殖行为。这些结果表明，可以通过控制材料表面特性引起的吸附蛋白质结构变化的分子相互作用，间接调节细胞反应。
