The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou 51642, China.
Gene. 2020 Sep 25;756:144921. doi: 10.1016/j.gene.2020.144921. Epub 2020 Jun 25.
Lilium 'Siberia' is a perennial herbaceous plant that is commercially significant because of its snowy white floral color and appealing scent which is mainly due to the presence of monoterpenes and benzoids compounds in floral volatile profile. In the current study, LoTPS5 was cloned and functionally characterized. Results revealed that LoTPS5 specifically generates squalene from FPP, whereas no product was produced when it was incubated with GPP or GGPP. The subcellular localization experiment showed that LoTPS5 was located in plastids. Furthermore, LoTPS5 showed its high expression in the leaf followed by petals and sepals of the flower. Moreover, the expression of LoTPS5 gradually increased from the bud stage and peak at the full-bloom stage. Besides, LoTPS5 showed a diurnal circadian rhythmic pattern with a peak in the afternoon (16:00) followed by deep night (24:00) and morning (8:00), respectively. LoTPS5 is highly responsive to mechanical wounding by rapidly elevating its mRNA transcript level. The current study will provide significant information for future studies of terpenoid and squalene biosynthesis in Lilium 'Siberia'.
西伯利亚百合是一种多年生草本植物，因其雪白的花色和诱人的香气而具有商业价值，主要归因于花朵挥发性成分中存在单萜和苯丙素化合物。在本研究中，克隆并功能表征了 LoTPS5。结果表明，LoTPS5 特异性地将 FPP 转化为角鲨烯，而当用 GPP 或 GGPP 孵育时则没有产物生成。亚细胞定位实验表明，LoTPS5 位于质体中。此外，LoTPS5 在叶片中的表达量最高，其次是花瓣和萼片。此外，LoTPS5 的表达量从芽期逐渐增加，在盛开期达到峰值。此外，LoTPS5 表现出昼夜节律性的节律模式，下午（16:00）出现峰值，随后是深夜（24:00）和清晨（8:00）。机械损伤会迅速提高 LoTPS5 的 mRNA 转录水平，使其对此高度响应。本研究将为西伯利亚百合萜类化合物和角鲨烯生物合成的未来研究提供重要信息。
