Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), Università di Padova, Agripolis, Viale dell'Università 16, 35020, Legnaro, Padova, Italy.
Biotechnol Adv. 2020 Sep-Oct;42:107579. doi: 10.1016/j.biotechadv.2020.107579. Epub 2020 Jun 25.
Recent advances in amylolytic strain engineering for starch-to-ethanol conversion have provided a platform for the development of raw starch consolidated bioprocessing (CBP) technologies. Several proof-of-concept studies identified improved enzyme combinations, alternative feedstocks and novel host strains for evaluation and application under fermentation conditions. However, further research efforts are required before this technology can be scaled up to an industrial level. In this review, different CBP approaches are defined and discussed, also highlighting the role of auxiliary enzymes for a supplemented CBP process. Various achievements in the development of amylolytic Saccharomyces cerevisiae strains for CBP of raw starch and the remaining challenges that need to be tackled/pursued to bring yeast raw starch CBP to industrial realization, are described. Looking towards the future, it provides potential solutions to develop more cost-effective processes that include cheaper substrates, integration of the 1G and 2G economies and implementing a biorefinery concept where high-value products are also derived from starchy substrates.
近年来，在用于淀粉到乙醇转化的淀粉分解菌株工程方面的进展为开发原始淀粉综合生物加工（CBP）技术提供了平台。一些概念验证研究确定了改进的酶组合、替代原料和新型宿主菌株，以便在发酵条件下进行评估和应用。然而，在这项技术能够扩展到工业规模之前，还需要进一步的研究努力。在这篇综述中，定义和讨论了不同的 CBP 方法，并强调了辅助酶在补充 CBP 过程中的作用。描述了开发用于原始淀粉 CBP 的淀粉分解酿酒酵母菌株的各种成就，以及为将酵母原始淀粉 CBP 推向工业实现仍需解决/追求的挑战。展望未来，它为开发更具成本效益的工艺提供了潜在的解决方案，包括更便宜的底物、整合 1G 和 2G 经济以及实施生物精炼厂概念，从淀粉质底物中获得高价值产品。
