Department of Intensive Medicine, Hebei General Hospital, 348 Heping Road, Shijiazhuang, Hebei, 050011, PR China.
Department of Pain, Hebei General Hospital, 348 Heping Road, Shijiazhuang, Hebei, 050011, PR China.
Biomed Pharmacother. 2020 Sep;129:110343. doi: 10.1016/j.biopha.2020.110343. Epub 2020 Jun 25.
The study investigated the effect of soluble dietary fiber supplementation on gut microbiota, mucosal barrier function, inflammatory markers, and survival in a murine model of sepsis established by cecal ligation and puncture (CLP). The expression of muc2 was down-regulated in septic mice. However, muc2 expression was significantly increased in the septic mice that received soluble dietary fiber. Survival was also improved in the septic mice treated with dietary fiber. The survival benefit was associated with decreased serum concentrations of pro-inflammatory cytokines and reduced neutrophil infiltration in the gut. Furthermore, the mucus thickness, inflammatory response, and degree of gut injury were remarkably alleviated in septic mice treated with soluble dietary fiber, while it was aggravated in septic mice treated with antibiotics in addition to the soluble fiber. Bacterial 16S ribosomal RNA gene sequencing also showed that fiber supplementation caused an increase in the relative abundance of probiotics, commonly associated with metabolic health. Administration of antibiotics to mice fed fiber diet negated the enrichment of probiotics as well as the survival benefit, following cecal ligation and puncture. Dietary supplementation with cellulose offers a microbe-mediated survival advantage in a murine model of sepsis. Improved understanding of the connection between diet, microbiota, and systemic illness may yield new therapeutic strategies for patients with sepsis.
该研究通过盲肠结扎穿刺（CLP）建立的脓毒症小鼠模型，调查了可溶性膳食纤维补充对肠道微生物群、黏膜屏障功能、炎症标志物和生存的影响。脓毒症小鼠的 muc2 表达下调。然而，接受可溶性膳食纤维的脓毒症小鼠的 muc2 表达显著增加。膳食纤维治疗的脓毒症小鼠的生存率也有所提高。生存获益与血清中促炎细胞因子浓度降低和肠道中性粒细胞浸润减少有关。此外，在接受可溶性膳食纤维治疗的脓毒症小鼠中，黏液厚度、炎症反应和肠道损伤程度明显减轻，而在接受抗生素治疗的脓毒症小鼠中则加重。细菌 16S 核糖体 RNA 基因测序还表明，纤维补充剂增加了与代谢健康相关的益生菌的相对丰度。在给予纤维饮食的小鼠中给予抗生素，会消除益生菌的富集以及在盲肠结扎和穿刺后的生存获益。在脓毒症小鼠模型中，纤维素的饮食补充提供了一种微生物介导的生存优势。对饮食、微生物群和全身疾病之间联系的深入了解，可能为脓毒症患者提供新的治疗策略。
