Jiu Guniang, Dao Riao, Wu Dongxing, Hung Wang, Jin Haburi, Li Li, Fu Xiquan, Sa Chula
Mongolian Medical College, Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia, China.
Affiliated Hospital of Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia, China.
Evid Based Complement Alternat Med. 2022 May 30;2022:7255780. doi: 10.1155/2022/7255780. eCollection 2022.
Heyi disease, Xila disease, and Badagan disease are three common diseases in Mongolian medicine. The changes in intestinal microbiota may be associated with the occurrence, development, and treatment of these diseases. This study aimed to investigate the effects of herbal treatment on intestinal microbiota and serum metabolites in rats with these three diseases.
Firstly, Heyi, Xila, and Badagan disease model rats were established by environmental, diet, and drug intervention. Then, 16S rRNA gene sequencing and metabolomics analysis were used to analyze the changes in intestinal microbiota and serum metabolites after treatment. PICRUSt analysis was applied to predict the potential functions of intestinal microbiota, and OPLS-DA multivariate model was applied to screen differential serum metabolites.
16S rRNA gene sequencing showed that herbal treatment significantly increased the species diversity and changed the composition of intestinal microbiota in Heyi disease and Xila disease rats. After treatment, there were 10, 9, and 3 bacterial biomarkers that were increased in Heyi, Xila, and Badagan disease rats, respectively. In the Heyi disease model, treatment resulted in 45 differential serum metabolites, involving 4 pathways. In the Badagan disease model, treatment resulted in 62 differential serum metabolites, involving 4 pathways. However, there was no significant difference in serum metabolites between TreatB and ConB in the Xila disease model.
Herbal treatment significantly changed the intestinal microbiota and serum metabolites of rats with three Mongolian medicine diseases.
赫依病、希拉病和巴达干病是蒙医中的三种常见疾病。肠道微生物群的变化可能与这些疾病的发生、发展及治疗相关。本研究旨在探讨中药治疗对患有这三种疾病的大鼠肠道微生物群和血清代谢物的影响。
首先，通过环境、饮食和药物干预建立赫依、希拉和巴达干病模型大鼠。然后，采用16S rRNA基因测序和代谢组学分析来分析治疗后肠道微生物群和血清代谢物的变化。应用PICRUSt分析预测肠道微生物群的潜在功能，并应用OPLS-DA多元模型筛选差异血清代谢物。
16S rRNA基因测序显示，中药治疗显著增加了赫依病和希拉病大鼠肠道微生物群的物种多样性并改变了其组成。治疗后，赫依、希拉和巴达干病大鼠分别有10种、9种和3种细菌生物标志物增加。在赫依病模型中，治疗导致45种差异血清代谢物，涉及4条通路。在巴达干病模型中，治疗导致62种差异血清代谢物，涉及4条通路。然而，在希拉病模型中，治疗组（TreatB）和对照组（ConB）之间的血清代谢物没有显著差异。
中药治疗显著改变了患有三种蒙医疾病大鼠的肠道微生物群和血清代谢物。
