Bai Chyi-Huey, Alizargar Javad, Peng Ching-Yi, Wu Jia-Ping
Department of Public Health, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Research Center for Healthcare Industry Innovation, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan.
Chin J Physiol. 2020 May-Jun;63(3):101-112. doi: 10.4103/CJP.CJP_95_19.
Obese sarcopenia is a progressive loss of skeletal muscle mass and strength with increases in adipocytes. The aim of this study was to investigate the effects of combination of exercise training and resveratrol on the pathological pathway from obesity to sarcopenia, and potential therapy for skeletal muscle declines in physical function. Two animal models were experimented: (1) C57BL/6J male mice were fed either a high-fat diet (HFD) for 8 weeks to induce obesity and resveratrol (low-, middle-, and high-dose) for 4 weeks. (2) senescence-accelerated mouse prone 8 (SAMP8) mice with sarcopenia were used. Skeletal muscle function of SAMP8 mice expressed an age-associated decline. In SAMP8 mice, resveratrol (150 mg/Kg BW, daily) was administered by oral gavage two times a week for 1 month of the experimental period. Exercise training based on adaptations in the muscle is training twice a week for 4 weeks. SAMP8 mouse skeletal muscle in each group was analyzed by H and E staining, transferase dUTP nick end labeling, and Western blot analysis. Mitochondrial function expression, apoptosis and relative hypertrophy signaling in HFD-induced obesity mice and SAMP8 mice were determined by Western blot analysis. Results of the present study indicate that effect of resveratrol on skeletal muscles of HFD-induced obesity mice is linked to an increase in Bcl-2 and phosphatidylinositol 3 kinase/AKT expressions. On the other hand, resveratrol, and its combination with exercise training, attenuate the aging-related mitochondrial dysfunction involving Bad, caspase 3, and interleukin-6 expressions in SAMP8 mice. Combination of exercise training and resveratrol induced hypertrophy in skeletal muscles of sarcopenia SAMP8 mice. Therefore, we suggest combination of exercise training and resveratrol as a therapeutic potential in obese sarcopenia.
肥胖性肌肉减少症是一种骨骼肌质量和力量逐渐丧失且脂肪细胞增加的病症。本研究的目的是探讨运动训练与白藜芦醇联合使用对从肥胖到肌肉减少症的病理途径的影响，以及对骨骼肌身体功能下降的潜在治疗方法。实验采用了两种动物模型：（1）将C57BL/6J雄性小鼠喂食高脂饮食（HFD）8周以诱导肥胖，并给予白藜芦醇（低、中、高剂量）4周。（2）使用患有肌肉减少症的衰老加速小鼠8型（SAMP8）小鼠。SAMP8小鼠的骨骼肌功能呈现与年龄相关的下降。在SAMP8小鼠中，在实验期的1个月内，每周通过口服灌胃给予白藜芦醇（150mg/Kg体重，每日）两次。基于肌肉适应性的运动训练为每周训练两次，共4周。通过苏木精和伊红染色、转移酶dUTP缺口末端标记和蛋白质免疫印迹分析对每组SAMP8小鼠的骨骼肌进行分析。通过蛋白质免疫印迹分析测定高脂饮食诱导的肥胖小鼠和SAMP8小鼠中的线粒体功能表达、细胞凋亡和相对肥大信号。本研究结果表明，白藜芦醇对高脂饮食诱导的肥胖小鼠骨骼肌的作用与Bcl-2和磷脂酰肌醇3激酶/AKT表达的增加有关。另一方面，白藜芦醇及其与运动训练的联合使用可减轻SAMP8小鼠中与衰老相关的线粒体功能障碍，涉及Bad、半胱天冬酶3和白细胞介素-6的表达。运动训练和白藜芦醇的联合使用可诱导肌肉减少症SAMP8小鼠骨骼肌肥大。因此，我们建议运动训练和白藜芦醇联合使用对肥胖性肌肉减少症具有治疗潜力。
