Tokarev Yuri S, Malysh Svetlana M, Volodartseva Yuliya V, Gerus Aleksei V, Berezin Mikhail V
Laboratory of Microbiological Control, All-Russian Institute of Plant Protection, St. Petersburg, Russian Federation,
Laboratory of Microbiological Control, All-Russian Institute of Plant Protection, St. Petersburg, Russian Federation.
Intervirology. 2019;62(5-6):222-226. doi: 10.1159/000508839. Epub 2020 Jun 26.
Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90-100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a non-structural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870).
暗黑鳃金龟是一种热带拟步甲，被广泛用于商业大规模昆虫养殖。疾病爆发可能会导致养殖的昆虫全部死亡。在本文中，获取了患病的暗黑鳃金龟样本，并用于建立实验室模型，因为发现它们对来自另一个来源的同种昆虫幼虫具有传染性。中年幼虫大约需要1个月才会出现急性疾病症状，而用较年幼的幼虫进行感染时，所需时间大约是前者的两倍。受感染的幼虫很快死亡，几天内多达90%-100%的群体可能会死亡。使用针对编码非结构蛋白（ORF3）的基因的简并引物进行PCR检测，在健康和患病的幼虫中均检测到一种病毒。测序的基因片段（Genbank登录号#MN732869）证实该病毒属于浓核病毒科，与德国小蠊浓核病毒样病毒（#JQ320376）的最大相似度为97.2%，与德国小蠊浓核病毒（#AY189948）的相似度为66.2%。来自一个没有疾病症状的独立群体的暗黑鳃金龟幼虫的基因组DNA样本对这种病毒也呈阳性，其基因型略有不同（与暗黑鳃金龟浓核病毒的前一个序列的序列相似度为99.7%）（#MN732870）。
