Fang Ao, Hao Zhiyan, Wang Ling, Li Dichen, He Jiankang, Gao Lin, Mao Xinggang, Paz Rubén
School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, ShaanXi 710054, China.
State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, ShaanXi 710054, China.
Int J Bioprint. 2019 Jul 30;5(2):235. doi: 10.18063/ijb.v5i2.235. eCollection 2019.
The trauma of central nervous system (CNS) can lead to glial scar, and it can limit the regeneration of neurons at the injured area, which is considered to be a major factor affecting the functional recovery of patients with CNS injury. At present, the study of the glial scar model is still limited to two-dimensional culture, and the state of the scar cannot be well mimicked. Therefore, we use a collagen gel and astrocytes to construct a three-dimensional (3D) model to mimic natural glial scar tissue. The effects of concentration changes of astrocytes on cell morphology, proliferation, and tissue performance were investigated. After 8 days of culture , the results showed that the tissue model contracted, with a measured shrinkage rate of 4.5%, and the compressive elastic modulus increased to nearly 4 times. Moreover, the astrocytes of the 3D tissue model have the ability of proliferation, hyperplasia, and formation of scar clusters. It indicates that the model we constructed has the characteristics of glial scar tissue to some extent and can provide an model for the research of glial scar and brain diseases.
中枢神经系统（CNS）创伤可导致胶质瘢痕形成，且会限制损伤区域神经元的再生，这被认为是影响CNS损伤患者功能恢复的主要因素。目前，胶质瘢痕模型的研究仍局限于二维培养，无法很好地模拟瘢痕状态。因此，我们使用胶原凝胶和星形胶质细胞构建三维（3D）模型以模拟天然胶质瘢痕组织。研究了星形胶质细胞浓度变化对细胞形态、增殖及组织性能的影响。培养8天后，结果显示组织模型发生收缩，测得收缩率为4.5%，压缩弹性模量增加至近4倍。此外，3D组织模型中的星形胶质细胞具有增殖、增生及形成瘢痕簇的能力。这表明我们构建的模型在一定程度上具有胶质瘢痕组织的特征，可为胶质瘢痕及脑部疾病的研究提供模型。
