Zhan Yuanlong, Zhao Ruiying, Cui Hongliang, Li Huatai, Song Zhifeng, Liu Changli
College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jun 25;36(6):1101-1112. doi: 10.13345/j.cjb.190398.
As an important platform compound, 3-hydroxypropionic acid (3-HP) can be used as a substrate to synthesize a variety of biological products with commercial potential. The titer of 3-HP by wild-type bacteria is low, which severely limits the large-scale application and production of 3-HP. By modifying the genes related to the metabolic pathway, engineered bacteria using cheap substrates as carbon sources are constructed, the aim of reducing production cost and increasing output is realized. In this paper, the recent progress in the synthesis of 3-HP by metabolic engineering at home and abroad is reviewed. The advantages and disadvantages of glycerol pathway, malonyl-CoA pathway and beta-alanine pathway for synthesis of 3-HP are also summarized and analyzed, and the future development of 3-HP is prospected.
作为一种重要的平台化合物，3-羟基丙酸（3-HP）可作为底物用于合成多种具有商业潜力的生物产品。野生型细菌生产3-HP的效价较低，这严重限制了3-HP的大规模应用和生产。通过修饰与代谢途径相关的基因，构建了以廉价底物为碳源的工程菌，实现了降低生产成本和提高产量的目标。本文综述了国内外利用代谢工程合成3-羟基丙酸的研究进展。总结并分析了甘油途径、丙二酰辅酶A途径和β-丙氨酸途径合成3-羟基丙酸的优缺点，并对3-羟基丙酸的未来发展进行了展望。
