Chen Yue, Li Qin, Zhang Jie, Gu Rui, Li Kai, Zhao Gang, Yuan Hang, Feng Tianyu, Ou Deqiong, Lin Ping
Lab of Experimental Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2020 Jun 25;37(3):469-479. doi: 10.7507/1001-5515.202004064.
Tripartite motif 5 (TRIM5) plays a significant function in autophagy and involves in immune and tumor processes. While the function of TRIM5 remains poorly understood in glioma. We purpose to evaluate the possible prognostic role of TRIM5 in glioma via bioinformatics analyses. The database clinical samples of glioma in this study included low grade glioma (LGG) and glioblastoma multiforme (GBM). TRIM5 expression in glioma tissues were explored in Oncomine, GEPIA and The Cancer Genome Atlas (TCGA) databases. Survival analysis and the multivariate Cox regression analysis of TRIM5 based on TCGA were used to evaluate the prognostic role of TRIM5. The protein networks of TRIM5 was detected by STRING database. KEGG enrichment analyses were performed to predict the potential molecular pathways of TRIM5 in glioma. In addition, immune infiltration analysis was conducted by CIBERSORT and TIMER databases. We found that TRIM5 was strongly increased in glioma samples compared with normal samples in Oncomine, GEPIA and TCGA databases. Higher TRIM5 was significantly contributed to worse overall survival (OS) in LGG+GBM patients and LGG patients, while was no correlated with OS of GBM patients. Interaction networks analysis identified that IRF3, IRF7, OAS1, OAS2, OAS3, OASL, GBP1, PML, BTBD1 and BTBD2 proteins were contacted with TRIM5. Moreover, KEGG revealed that apoptosis and cancer- and immune-related pathways were enriched with elevated TRIM5. Specifically, TRIM5 could influence the immune infiltration levels, such as activated NK cells, monocytes, activated mast cells and macrophages in glioma. In conclusion, our data indicated that TRIM5 was upregulated in glioma tissues and associated with poor prognosis and immune infiltration. TRIM5 may be acted as a biomarker in prognosis and immunotherapy guidance of glioma.
三联基序蛋白5（TRIM5）在自噬中发挥重要作用，并参与免疫和肿瘤进程。然而，TRIM5在胶质瘤中的功能仍知之甚少。我们旨在通过生物信息学分析评估TRIM5在胶质瘤中可能的预后作用。本研究中胶质瘤的数据库临床样本包括低级别胶质瘤（LGG）和多形性胶质母细胞瘤（GBM）。在Oncomine、GEPIA和癌症基因组图谱（TCGA）数据库中探究了TRIM5在胶质瘤组织中的表达。基于TCGA对TRIM5进行生存分析和多变量Cox回归分析，以评估TRIM5的预后作用。通过STRING数据库检测TRIM5的蛋白质网络。进行KEGG富集分析以预测TRIM5在胶质瘤中的潜在分子途径。此外，通过CIBERSORT和TIMER数据库进行免疫浸润分析。我们发现在Oncomine、GEPIA和TCGA数据库中，与正常样本相比，胶质瘤样本中TRIM5显著升高。较高的TRIM5水平显著导致LGG + GBM患者和LGG患者的总生存期（OS）较差，而与GBM患者的OS无关。相互作用网络分析确定IRF3、IRF7、OAS1、OAS2、OAS3、OASL、GBP1、PML、BTBD1和BTBD2蛋白与TRIM5相互作用。此外，KEGG显示凋亡以及癌症和免疫相关途径在TRIM5升高时富集。具体而言，TRIM5可影响免疫浸润水平，如胶质瘤中的活化自然杀伤细胞、单核细胞、活化肥大细胞和巨噬细胞。总之，我们的数据表明TRIM5在胶质瘤组织中上调，与不良预后和免疫浸润相关。TRIM5可能作为胶质瘤预后和免疫治疗指导的生物标志物。
