Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
Biotechnol Appl Biochem. 2021 Jun;68(3):669-675. doi: 10.1002/bab.1978. Epub 2020 Aug 19.
C-phycocyanin (C-Pc), a photosynthetic pigment for use as a fluorescent indicator or in pharmaceutical, food, and cosmetic products, exists in a phycobilisome complex with allophycocyanin (APC), phycoerythrin (PE), and linker polypeptides. This heterogeneity makes it difficult to quantify phycobilisome composition in an ultraviolet-visible (UV-vis) spectrum. In this study, derivative analysis of UV-vis spectra was successfully applied to display the distinct wavelengths at which C-Pc, APC, and PE have maximal peaks. In all samples, C-Pc of the largest portion had a "zero-crossing" first order, APC did not have a zero-crossing first order, and PE did not have first derivative for zero crossing or local minimum from the 500 and 700 nm, respectively. The results show that derivative analyses coupled with signal smoothing can be applied to elucidate the composition of phycobilisome under various conditions including purification and environment.
藻蓝蛋白（C-Pc）是一种用于荧光指示剂或药物、食品和化妆品产品的光合色素，存在于藻胆体复合物中，与别藻蓝蛋白（APC）、藻红蛋白（PE）和连接多肽一起。这种异质性使得难以在紫外可见（UV-vis）光谱中定量藻胆体的组成。在这项研究中，成功地应用了紫外可见光谱的导数分析来显示 C-Pc、APC 和 PE 具有最大峰的不同波长。在所有样品中，最大部分的 C-Pc 具有“零交叉”一阶导数，APC 没有零交叉一阶导数，PE 没有从 500nm 和 700nm 开始的一阶导数的零交叉或局部最小值。结果表明，导数分析与信号平滑相结合，可以应用于阐明各种条件下藻胆体的组成，包括纯化和环境。
