通过分光光度法测定螺旋藻（节旋藻）补充剂和原料中藻蓝蛋白和别藻蓝蛋白的方法的单实验室验证。

Single-laboratory validation of a method for the determination of c-phycocyanin and allophycocyanin in Spirulina (Arthrospira) supplements and raw materials by spectrophotometry.

作者信息

Yoshikawa Noritaka, Belay Amha

机构信息

DIC LIFETEC Co., Ltd, Technical Department 12, Yawata-kaigandori, Ichihara, Chiba 290-8585, Japan.

出版信息

J AOAC Int. 2008 May-Jun;91(3):524-9.

Abstract

A single-laboratory validation study was conducted for a 2-wavelength spectrophotometric method for the determination of c-phycocyanin (cPC) and allophycocyanin (aPC) in Spirulina supplements and raw materials. The absorption maxima of cPC and aPC are at 620 and 650 nm, respectively. The concentrations of the analytes were calculated from measurement of absorbance at 2 wavelengths. This method provided linear responses for cPC and aPC in the range of 25-250 microg/mL. Duplicate determinations of cPC and aPC in 4 different test materials on 5 different days resulted in relative standard deviations of 0.3-1.0 and 1.0-1.5% for cPC and aPC, respectively. Recoveries were 99.2-102.4 and 100.0-104.0% for cPC and aPC, respectively. The results were satisfactory for the determination of cPC and aPC in Spirulina supplements.

摘要

对一种用于测定螺旋藻补充剂和原料中藻蓝蛋白（cPC）和别藻蓝蛋白（aPC）的双波长分光光度法进行了单实验室验证研究。cPC和aPC的最大吸收波长分别为620和650nm。通过测量两个波长处的吸光度来计算分析物的浓度。该方法在25 - 250μg/mL范围内对cPC和aPC提供线性响应。在5个不同日期对4种不同测试材料中的cPC和aPC进行重复测定，cPC和aPC的相对标准偏差分别为0.3 - 1.0%和1.0 - 1.5%。cPC和aPC的回收率分别为99.2 - 102.4%和100.0 - 104.0%。该结果对于螺旋藻补充剂中cPC和aPC的测定是令人满意的。