Wagley Pravin K, Williamson John, Skwarzynska Daria, Kapur Jaideep, Burnsed Jennifer
Department of Pediatrics, University of Virginia; Department of Neurology, University of Virginia.
Department of Neurology, University of Virginia.
J Vis Exp. 2020 Jun 11(160). doi: 10.3791/61346.
Hypoxia ischemia is the most common cause of neonatal seizures. Animal models are crucial for understanding the mechanisms and physiology underlying neonatal seizures and hypoxia ischemia. This manuscript describes a method for continuous video electroencephalogram (EEG) monitoring in neonatal mice to detect seizures and analyze EEG background during hypoxia ischemia. Use of video and EEG in conjunction allows description of seizure semiology and confirmation of seizures. This method also allows analysis of power spectrograms and EEG background pattern trends over the experimental time period. In this hypoxia ischemia model, the method allows EEG recording prior to injury to obtain a normative baseline and during injury and recovery. Total monitoring time is limited by the inability to separate pups from the mother for longer than four hours. Although, we have used a model of hypoxic-ischemic seizures in this manuscript, this method for neonatal video EEG monitoring could be applied to diverse disease and seizure models in rodents.
缺氧缺血是新生儿惊厥最常见的原因。动物模型对于理解新生儿惊厥和缺氧缺血的潜在机制及生理学至关重要。本手稿描述了一种在新生小鼠中进行连续视频脑电图（EEG）监测的方法，以检测惊厥并分析缺氧缺血期间的EEG背景。结合使用视频和EEG可以描述惊厥症状学并确认惊厥。该方法还允许在实验时间段内分析功率谱图和EEG背景模式趋势。在这个缺氧缺血模型中，该方法允许在损伤前进行EEG记录以获得正常基线，并在损伤和恢复期间进行记录。由于无法将幼崽与母亲分开超过四小时，总监测时间受到限制。尽管在本手稿中我们使用了缺氧缺血性惊厥模型，但这种新生儿视频EEG监测方法可应用于啮齿动物的多种疾病和惊厥模型。
