College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Int J Biol Macromol. 2020 Nov 1;162:935-945. doi: 10.1016/j.ijbiomac.2020.06.212. Epub 2020 Jun 26.
A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus. Monosaccharide composition analysis revealed that SLPC-1S was composed of galactose, glucose, arabinose and mannose in a molar ratio of 44.9:27.6:14.7:12.8. Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp. Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice. Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively. The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
从草菇的菌盖中纯化得到一种相对分子质量为 9.4 kDa 的杂多糖，命名为 SLPC-1S。单糖组成分析表明，SLPC-1S 由半乳糖、葡萄糖、阿拉伯糖和甘露糖组成，摩尔比为 44.9:27.6:14.7:12.8。结构表征表明，SLPC-1S 的主链主要由 1,3 连接的α-D-Galp、1,3 连接的β-D-Glcp 和 1,6 连接的β-D-Glcp 组成，支链主要由 1,3 连接的β-D-Glcp、1,3 连接的α-L-Arap、1,3 连接的α-D-Manp 和 T 连接的α-D-Galp 组成。此外，SLPC-1S 在链脲佐菌素诱导的糖尿病小鼠中表现出优异的抗糖尿病活性。通过 Western blot 和实时聚合酶链反应（RT-PCR）分别检测 NF-κB 和 Nrf2/HO-1 信号通路中的蛋白表达和 mRNA 水平。结果有力地证明，SLPC-1S 可以通过调节 Nrf2 介导的氧化应激和 NF-κB 介导的炎症反应，作为预防和治疗糖尿病的潜在药物。
