Laboratory of Nutrigenomics Studies, Health Science Department, Ribeirão Preto Medical School, Ribeirão Preto 14049-900, Brazil.
Department of Molecular Biology, São José do Rio Preto Medical School, São José do Rio Preto 15090-000, Brazil.
Int J Mol Sci. 2020 Jun 24;21(12):4476. doi: 10.3390/ijms21124476.
Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation of , an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 ± 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, the gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that the gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways.
体重调节和 Roux-en-Y 胃旁路术（RYGB）后的减重幅度可能由遗传决定。体重减轻干预措施（包括 RYGB）后，DNA 甲基化模式和一些基因的表达可以改变。本研究旨在评估肥胖和胰岛素相关基因 （ ）的基因表达和 DNA 甲基化在 RYGB 后的变化。从 13 名患有严重肥胖症的女性（35.9±9.2 岁）中采集手术前后的血液样本。通过基于微阵列的全基因组技术评估全血转录组和表观基因组模式。在 RYGB 的术前和术后期间共鉴定出 1966 个差异表达基因。从这些基因中，我们观察到与肥胖和胰岛素途径相关的基因在手术后上调。然后，选择基因进行进一步的 RT-qPCR 分析和胞嘧啶-鸟嘌呤核苷酸（CpG）位点甲基化评估。我们观察到 基因上调，并且肥胖症手术后六个 DNA 甲基化 CpG 位点的甲基化程度不同。总之，我们发现 RYGB 上调了与肥胖和胰岛素途径相关的基因。
