Ajitkumar P, Geier S S, Kesari K V, Borriello F, Nakagawa M, Bluestone J A, Saper M A, Wiley D C, Nathenson S G
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Cell. 1988 Jul 1;54(1):47-56. doi: 10.1016/0092-8674(88)90178-x.
Single amino acid substitutions at nine different positions on the H-2Kb molecules from in vitro-mutagenized, immunologically altered, somatic cell variants were correlated with their patterns of recognition by monoclonal antibodies (MAbs) and allogeneic cytotoxic T lymphocyte (CTL) clones. While MAbs were found to detect spatially discrete, domain-specific sites, CTLs interacted simultaneously with multiple residues on the alpha 1 and alpha 2 domains of the Kb molecule. The computer graphic three-dimensional Kb model structure showed that, of the seven CTL-specific residues analyzed, six residues were located on the alpha-helical regions of the two domains. Every CTL clone was found to interact with a distinct pattern of residues composed of a specific subset of the CTL-specific residues.
对体外诱变、免疫改变的体细胞变体的H-2Kb分子上九个不同位置的单个氨基酸取代，与其被单克隆抗体（MAb）和同种异体细胞毒性T淋巴细胞（CTL）克隆识别的模式进行了关联分析。虽然发现单克隆抗体可检测空间上离散的、结构域特异性位点，但CTL与Kb分子α1和α2结构域上的多个残基同时相互作用。计算机图形三维Kb模型结构显示，在分析的七个CTL特异性残基中，六个残基位于两个结构域的α螺旋区域。发现每个CTL克隆都与由CTL特异性残基的特定子集组成的独特残基模式相互作用。
