Key Laboratory of Tea Science of Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
Planta. 2020 Jun 29;252(1):10. doi: 10.1007/s00425-020-03407-0.
Transcriptomic studies in resistant and susceptible tea cultivars have been performed to reveal the different defense molecular mechanisms of tea after E. onukii feeding. The molecular mechanism by which tea plants respond to small green leafhopper Empoasca onukii (Matsuda) damage is unclear. Using the resistant tea plant cultivar Juyan (JY) and the susceptible tea plant cultivar Enbiao (EB) as materials, this study performed RNA-seq on tea leaf samples collected at three time points (6 h, 12 h, 24 h) during exposure of the plants to leafhopper to reveal the molecular mechanisms that are activated in susceptible and resistant tea plant cultivars in response to leafhopper damage. The numbers of DEGs in the susceptible tea cultivar during early (6 h) and late (24 h) stages of leafhopper induction were higher than those in the resistant cultivar at the same time points. The stress responses to leafhopper were most intense at 12 h in both tea cultivars. Pathway enrichment analysis showed that most up-regulated DEGs and their related metabolic pathways were similar in the two tea cultivars. However, during the early stage of leafhopper induction (6 h), jasmonic acid (JA)-related genes were significantly up-regulated in the resistant cultivar. The terpenoid biosynthetic pathway and the α-linolenic acid metabolic pathway were activated earlier in the resistant cultivar and remained activated until the late stage of leafhopper damage. Our results confirmed that after leafhopper damage, the resistant tea cultivar activated its defense responses earlier than the susceptible cultivar, and these defense responses were mainly related to terpenoid metabolism and JA biosynthetic pathway. The results provide important clues for further studies on resistance strategy of tea plants to pest.
转录组研究在抗性和敏感茶树品种进行了揭示不同的防御分子机制的茶树后 E. onukii 喂养。茶树对小绿叶蝉 Empoasca onukii (松田) 损害的分子机制尚不清楚。使用抗性茶树品种 Juyan (JY) 和敏感茶树品种 Enbiao (EB) 作为材料，本研究对暴露于叶蝉后不同时间点(6 h、12 h、24 h)的茶树叶片样本进行 RNA-seq 分析，揭示了敏感和抗性茶树品种在受到叶蝉伤害时激活的分子机制。在早期(6 h)和晚期(24 h)叶蝉诱导阶段，敏感茶树品种中差异表达基因的数量高于同一时间点的抗性品种。两种茶树在 12 h 时对叶蝉的应激反应最为强烈。通路富集分析表明，两种茶树中上调的差异表达基因及其相关代谢通路大多相似。然而，在叶蝉诱导的早期阶段(6 h)，抗性品种中与茉莉酸(JA)相关的基因显著上调。萜类生物合成途径和α-亚麻酸代谢途径在抗性品种中更早被激活，并一直持续到叶蝉损伤的晚期。研究结果证实，在受到叶蝉伤害后，抗性茶树品种比敏感品种更早地激活其防御反应，这些防御反应主要与萜类代谢和 JA 生物合成途径有关。研究结果为进一步研究茶树对害虫的抗性策略提供了重要线索。
