O'Neill H C, Parish C R
Department of Experimental Pathology, John Curtin School of Medical Research, Australian National University, Canberra.
Immunology. 1988 May;64(1):181-4.
A range of monosaccharides has been tested for their capacity to influence the induction and effector function of alloreactive cytotoxic T (Tc) cells. Strain-specific differences in the capacity of monosaccharides to inhibit Tc cell induction have been demonstrated. Monosaccharides can also inhibit effector function of target cell lysis, but this could only be demonstrated by assessing the effect of sugars added to limiting dilution cultures of alloantigen-stimulated T cells. B10.A(4R) anti-BALB/c Tc cells have been reproducibly inhibited by D-glucosamine and D-galactosamine, as well as D-galacturonic acid, at both the induction and effector phases of the Tc cell response. Analysis of monosaccharide inhibition of cytotoxicity in limiting dilution cultures has confirmed that D-glucosamine is the most effective inhibitor of B10.A(4R) anti-BALB/c Tc cells, while D-galactosamine and D-galacturonic acid inhibit cytotoxicity in only some limiting dilution wells. Analysis of several B10.A(4R) anti-BALB/c Tc cell clones has revealed at least two different 'clone-specific' patterns of inhibition by D-glucose, D-glucuronic acid and D-galacturonic acid. Since Tc cell recognition of antigen is generally specific for class I major histocompatibility complex (MHC) antigens, this data implicates a role for MHC-associated carbohydrate structures expressed by target cells in T-lymphocyte interactions with antigen.
已对一系列单糖影响同种异体反应性细胞毒性T（Tc）细胞诱导和效应功能的能力进行了测试。已证明单糖抑制Tc细胞诱导能力存在品系特异性差异。单糖还可抑制靶细胞裂解的效应功能，但这只能通过评估添加到同种异体抗原刺激的T细胞有限稀释培养物中的糖类的作用来证明。在Tc细胞反应的诱导期和效应期，D-葡糖胺、D-半乳糖胺以及D-半乳糖醛酸均可重复性地抑制B10.A(4R)抗BALB/c Tc细胞。对有限稀释培养物中细胞毒性的单糖抑制作用分析证实，D-葡糖胺是B10.A(4R)抗BALB/c Tc细胞最有效的抑制剂，而D-半乳糖胺和D-半乳糖醛酸仅在某些有限稀释孔中抑制细胞毒性。对几个B10.A(4R)抗BALB/c Tc细胞克隆的分析揭示了D-葡萄糖、D-葡糖醛酸和D-半乳糖醛酸至少两种不同的“克隆特异性”抑制模式。由于Tc细胞对抗原的识别通常对I类主要组织相容性复合体（MHC）抗原具有特异性，因此该数据表明靶细胞表达的MHC相关碳水化合物结构在T淋巴细胞与抗原的相互作用中发挥作用。
