Bhardwaj Sonam, Kumar Pradeep, Jerome Andonissamy, Ravesh Suman, Patil Chandrashekhar, Singh Pawan, Lailer Puran Chand
Animal Physiology and Reproduction Division, ICAR- Central Institute for Research on Buffaloes, Hisar, India.
Livestock Production and Management Section, ICAR- National Dairy Research Institute, Karnal, India.
Reprod Domest Anim. 2020 Sep;55(9):1190-1201. doi: 10.1111/rda.13761. Epub 2020 Jul 18.
The study was designed to decipher the inter-relationship between peripheral hormones (kisspeptin and testosterone), sexual behaviour and seminal variables of Murrah buffalo bulls (n = 134). In this study, we recorded that 13%, 37%, 40%, 6% and 4% Murrah buffalo bulls had reaction time of <30, 31-60, 61-180, 181-300 and >300 s, respectively. Further, it was observed that 4%, 85% and 10% buffalo bulls were sexually aggressive, active and dull, respectively, during semen collection. The courtship behaviour was not found to be desirable for the bulls used for the semen collection. Mean of ejaculate volume, sperm concentration and mass motility (0-5 scale) were 3.57 ml, 977.11 million/ml, 2.7, respectively. Correlation studies revealed that the reaction time was positively correlated with courtship behaviour and body weight, and negatively correlated with sexual aggressiveness and sperm concentration. Serum kisspeptin in buffalo bulls, measured for the first time, was found to 3.8 ± 0.7 ng/ml. Serum kisspeptin and testosterone level are negatively correlated to each other and kisspeptin level influenced the sexual behaviour (reaction time, sexual aggressiveness and penile erection) of study bulls. Serum kisspeptin was higher in the buffalo bulls with higher sperm concentration indicating its role in spermatogenesis. In conclusion, for the first time basic information related to sexual behaviour of Murrah buffalo bulls in large population along with its inter-relationship with peripheral hormones (kisspeptin and testosterone) has been documented.
本研究旨在解读穆拉水牛公牛（n = 134）外周激素（亲吻素和睾酮）、性行为与精液变量之间的相互关系。在本研究中，我们记录到，穆拉水牛公牛的反应时间<30秒、31 - 60秒、61 - 180秒、181 - 300秒和>300秒的比例分别为13%、37%、40%、6%和4%。此外，观察到在采精过程中，分别有4%、85%和10%的水牛公牛具有性攻击性、性行为活跃和性行为迟钝。未发现用于采精的公牛的求偶行为令人满意。射精量、精子浓度和活力（0 - 5级）的平均值分别为3.57毫升、9.7711亿/毫升、2.7。相关性研究表明，反应时间与求偶行为和体重呈正相关，与性攻击性和精子浓度呈负相关。首次测定水牛公牛血清亲吻素水平为3.8±0.7纳克/毫升。血清亲吻素和睾酮水平呈负相关，亲吻素水平影响了受试公牛的性行为（反应时间、性攻击性和阴茎勃起）。精子浓度较高的水牛公牛血清亲吻素水平也较高，表明其在精子发生过程中的作用。总之，首次记录了大量穆拉水牛公牛性行为的基本信息及其与外周激素（亲吻素和睾酮）的相互关系。
