Rodrigues M M, Xavier M T, Mendonça-Previato L, Barcinski M A
Discipline of Parasitology, Escola Paulista de Medicina, Sao Paulo, Brazil.
Infect Immun. 1988 Jul;56(7):1766-70. doi: 10.1128/iai.56.7.1766-1770.1988.
Recently, a glycoprotein fraction, designated gp10/20, purified from Leishmania mexicana amazonensis was shown to induce a cellular immune response mediated by murine L3T4+ T lymphocytes. This fact led us to pursue further the characterization of this fraction. The present study demonstrated that gp10/20 is a degradation product of a 17-kilodalton antigen present in promastigotes and amastigotes of L. mexicana amazonensis. This antigen was easily detected in promastigotes of L. mexicana mexicana, L. donovani, L. chagasi, L. major, and L. tropica. However, culture forms of L. braziliensis complex expressed either low amounts of the 17-kilodalton antigen or an antigenically unrelated antigen. The recognition of gp10/20 by several serum samples of patients with kala-azar was also shown.
最近，从亚马逊利什曼原虫中纯化得到的一种糖蛋白组分，命名为gp10/20，被证明可诱导由小鼠L3T4+ T淋巴细胞介导的细胞免疫反应。这一事实促使我们进一步对该组分进行特性研究。本研究表明，gp10/20是存在于亚马逊利什曼原虫前鞭毛体和无鞭毛体中的一种17千道尔顿抗原的降解产物。在墨西哥利什曼原虫、杜氏利什曼原虫、恰加斯利什曼原虫、硕大利什曼原虫和热带利什曼原虫的前鞭毛体中很容易检测到这种抗原。然而，巴西利什曼原虫复合体的培养形式要么表达少量的17千道尔顿抗原，要么表达一种抗原性无关的抗原。也显示了黑热病患者的几份血清样本对gp10/20的识别。
