Carvalho E M, Teixeira R S, Johnson W D
Infect Immun. 1981 Aug;33(2):498-500. doi: 10.1128/iai.33.2.498-500.1981.
Cell-mediated immunity was assessed in 14 Brazilian patients with acute untreated American visceral leishmaniasis (AVL) and in 11 healthy patients successfully treated 1 to 14 years previously. The diagnosis of AVL was established by demonstration of leishmania in bone marrow aspirates. The responsiveness of peripheral blood mononuclear cells to Leishmania chagasi antigens and phytohemagglutinin was studied in vitro. Soluble preparations of L. chagasi antigens were obtained from frozen-thawed promastigote cultures. L. chagasi antigen-stimulated lymphocytes from untreated AVL patients were unresponsiveness and incorporated a mean of 1.2 +/- 0.5 X 10(-3) cpm after a [3H]thymidine pulse. The cured AVL patients had 19.1 +/- 7.2 cpm, and 15 normal control subjects had 0.8 +/- 0.1 cpm. There were no differences in the response of controls and either untreated or cured AVL patients to phytohemagglutinin stimulation. Three of four untreated AVL patients responded to L. chagasi antigens when studied 2 to 4 weeks after therapy. The impaired response of lymphocytes from untreated AVL patients could not be attributed to either reduced numbers of circulating T cells or the inhibitory effect of monocytes or serum factors.
对14例未经治疗的急性美洲内脏利什曼病（AVL）巴西患者以及11例1至14年前成功治愈的健康患者进行了细胞介导免疫评估。通过在骨髓穿刺物中发现利什曼原虫来确诊AVL。体外研究了外周血单个核细胞对恰加斯利什曼原虫抗原和植物血凝素的反应性。恰加斯利什曼原虫抗原的可溶性制剂从冻融的前鞭毛体培养物中获得。未经治疗的AVL患者的恰加斯利什曼原虫抗原刺激的淋巴细胞无反应性，在[3H]胸腺嘧啶脉冲后平均掺入1.2 +/- 0.5 X 10（-3）cpm。治愈的AVL患者为19.1 +/- 7.2 cpm，15名正常对照受试者为0.8 +/- 0.1 cpm。对照组与未经治疗或治愈的AVL患者对植物血凝素刺激的反应没有差异。4例未经治疗的AVL患者中有3例在治疗后2至4周进行研究时对恰加斯利什曼原虫抗原有反应。未经治疗的AVL患者淋巴细胞反应受损不能归因于循环T细胞数量减少或单核细胞或血清因子的抑制作用。
