Department of Health Care Center, Beijing Friendship Hospital Medical, 12517Capital Medical University, Beijing, China.
Department of Pharmacy, 34706The First Affiliated Hospital of Nanhua University, Hengyang City, Hunan Province, China.
Hum Exp Toxicol. 2020 Dec;39(12):1596-1606. doi: 10.1177/0960327120934537. Epub 2020 Jun 30.
Monoterpenes present in the essential oils exhibit anti-inflammatory properties. In this study, we investigated the preventive effect of alpha-pinene (AP), a monoterpene, against isoproterenol (ISO)-induced myocardial infarction and inflammation in Wistar rats. Male Wistar rats were pretreated with AP (50 mg/kg body weight (bw)) administration for 21 days and ISO (85 mg/kg bw) was administered subcutaneously for last two consecutive days (20th day and 21st day). We noticed that there was an increased activity of cardiac marker enzymes in ISO-treated rats. We also observed that elevated levels of lipid peroxidative indices decreased activities of antioxidant status in plasma, erythrocyte, and heart tissue in ISO-induced rats. Furthermore, ISO-treated rats showed an increase in the levels of inflammatory mediators like tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the serum. Besides, we confirmed the upregulated expression of TNF-α, IL-6, and nuclear factor kappa-light-chain-enhancer of activated B cells in ISO-induced rat heart tissue. Conversely, we found that AP pretreatment significantly decreased levels of cardiac markers like serum cardiac troponin T and cardiac troponin I, lipid peroxidative markers, and restored antioxidants status in ISO-treated rats. Besides, AP administration attenuated ISO-induced inflammatory marker expression. The present findings demonstrated that AP significantly protects the myocardium and exerts cardioprotective and anti-inflammatory effects in experimental rats.
单萜类化合物存在于精油中，具有抗炎特性。在这项研究中，我们研究了单萜烯α-蒎烯 (AP) 对异丙肾上腺素 (ISO) 诱导的 Wistar 大鼠心肌梗死和炎症的预防作用。雄性 Wistar 大鼠用 AP（50mg/kg 体重）预处理 21 天，并用 ISO（85mg/kg bw）连续皮下注射 2 天（第 20 天和第 21 天）。我们注意到 ISO 处理的大鼠中心脏标记酶的活性增加。我们还观察到，血浆、红细胞和心脏组织中抗氧化状态的活性升高，脂质过氧化指标升高，ISO 诱导的大鼠中降低。此外，ISO 处理的大鼠血清中炎症介质如肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平增加。此外，我们证实 ISO 诱导的大鼠心脏组织中 TNF-α、IL-6 和核因子 kappa-轻链增强子的 B 细胞的表达上调。相反，我们发现 AP 预处理可显著降低 ISO 处理大鼠血清中心脏肌钙蛋白 T 和心脏肌钙蛋白 I、脂质过氧化标志物的水平，并恢复 ISO 处理大鼠的抗氧化状态。此外，AP 给药可减弱 ISO 诱导的炎症标志物的表达。本研究结果表明，AP 可显著保护心肌，对实验大鼠具有心脏保护和抗炎作用。
