Department of Cardiology, Xi'an Fourth Hospital, Xi'an, Shaanxi, China.
Department of Biochemistry and Biotechnology, 29895Annamalai University, Annamalai Nagar, Tamil Nadu, India.
Hum Exp Toxicol. 2021 Jun;40(6):915-927. doi: 10.1177/0960327120975131. Epub 2020 Nov 26.
This study aimed to investigate the antihyperlipidemic and anti-inflammatory effect of zingiberene (ZBN) on isoproterenol-(ISO) induced myocardial infarction in rats. ZBN (10 mg/kg b.wt.) was orally administered to rats for 21 days and ISO (85 mg/kg b.wt.) was subcutaneously injected into the rats at 24 h intervals for the last 2 consecutive days. We observed increased serum creatine kinase, creatine kinase-MB, cardiac troponin T, and I levels in ISO-treated MI rats. Conversely, ZBN oral administration significantly prevented in cardiac marker enzyme activities in ISO-mediated rats. We also noticed that ZBN oral administration prevented ISO-induced expression of lipid peroxidative markers, total cholesterol, triglycerides, phospholipids, free fatty acids, very-low-density lipoprotein cholesterol (VLDL-C), low-density lipoprotein cholesterol (LDL-C) to the normal basal level. Furthermore, ZBN restored ISO-mediated antioxidant status, increased level of high-density lipoprotein cholesterol (HDL-C), and tissue phospholipids to the near-normal levels. Besides, ZBN pre-treatment significantly reduced the level of inflammatory markers (TNF-α, IL-6, NF-κB, and IL-1β) in ISO-induced MI in rats. We noticed that ZBN pretreatment inhibited the pro-apoptotic proteins Bax and cytochrome c and increased the Bcl-2 expression in ISO induced rats. The gene expression profiling by qRT-PCR array illustrates that ZBN treatment prevents the ISO mediated activation of cardiac markers, inflammatory, and fibrosis-related genes in the heart tissue. Taken together, pre-treatment with ZBN attenuated ISO-induced MI resolved exhibits the anti-inflammatory and antiapoptotic effect.
本研究旨在探讨姜烯（ZBN）对异丙肾上腺素（ISO）诱导的大鼠心肌梗死的降脂和抗炎作用。ZBN（10mg/kg 体重）灌胃给药大鼠 21 天，最后连续 2 天，每隔 24 小时皮下注射 ISO（85mg/kg 体重）。我们观察到 ISO 处理的 MI 大鼠血清肌酸激酶、肌酸激酶-MB、心肌肌钙蛋白 T 和 I 水平升高。相反，ZBN 口服给药可显著防止 ISO 介导的大鼠心脏标志物酶活性。我们还注意到，ZBN 口服给药可防止 ISO 诱导的脂质过氧化标志物、总胆固醇、甘油三酯、磷脂、游离脂肪酸、极低密度脂蛋白胆固醇（VLDL-C）、低密度脂蛋白胆固醇（LDL-C）的表达升高到正常基础水平。此外，ZBN 恢复了 ISO 介导的抗氧化状态，将高密度脂蛋白胆固醇（HDL-C）和组织磷脂水平提高到接近正常水平。此外，ZBN 预处理可显著降低 ISO 诱导的 MI 大鼠炎症标志物（TNF-α、IL-6、NF-κB 和 IL-1β）的水平。我们注意到，ZBN 预处理可抑制 ISO 诱导的大鼠中促凋亡蛋白 Bax 和细胞色素 c 的表达，并增加 Bcl-2 的表达。qRT-PCR 阵列的基因表达谱表明，ZBN 预处理可防止 ISO 介导的心脏标志物、炎症和纤维化相关基因在心脏组织中的激活。综上所述，ZBN 预处理可减轻 ISO 诱导的 MI，表现出抗炎和抗凋亡作用。
