Department of Orthodontics, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
Iran J Immunol. 2020 Jun;17(2):137-143. doi: 10.22034/iji.2020.85915.1731.
IL-17 is reported to be associated with the pathophysiology of Orthodontic Tooth Movement (OTM) by affecting osteoclastogenesis.
To explore the changes of Th17 cytokines (IL-17, IL-23, and IL-27) expression and its correlation with receptor activator of nuclear factor kappa B ligand (RANKL) during orthodontic tooth movement.
Thirty patients who needed extraction of the first premolar during orthodontic treatment were included. The gingival crevicular fluid was sampled at the day of application (T0), one hour (T1), 24 hours (T2), one week (T3), four weeks (T4), and 12 weeks (T5) after the application of orthodontic force. The expression of Th17 cytokines and RANKL were measured by using enzyme-linked immunosorbent assay and, their correlations were assessed.
The levels of IL-17A, IL-17F, IL-23, and IL-27 at both tension and pressure sides of studied teeth at T2-T4 were significantly higher compared with that of T0 and T1. Moreover, the expression of IL-27 at both tension and pressure sides of studied teeth at T2-T4 was significantly lower compared with that of T0 and T1. At T5, IL-17A, IL-17F, IL-23, and IL-27 returned to the baseline level. For the control group, the cytokines were not significantly different at various time points. The expression of IL-17A, IL-17F, and IL-23 was positively correlated with RANKL expression at T2-T4, whereas the IL-27 was negatively correlated with RANKL expression at T2-T4.
This study provided preliminary evidence that Th17 cytokines may be involved in the regulation of OTM.
据报道，IL-17 通过影响破骨细胞生成与正畸牙齿移动（OTM）的病理生理学有关。
探讨正畸牙齿移动过程中 Th17 细胞因子（IL-17、IL-23 和 IL-27）表达的变化及其与核因子κB 受体激活剂配体（RANKL）的相关性。
纳入 30 名因正畸治疗需要拔除第一前磨牙的患者。在加力当天（T0）、加力后 1 小时（T1）、24 小时（T2）、1 周（T3）、4 周（T4）和 12 周（T5）时采集龈沟液样本。采用酶联免疫吸附试验检测 Th17 细胞因子和 RANKL 的表达，并评估它们之间的相关性。
研究牙齿的张力侧和压力侧在 T2-T4 时的 IL-17A、IL-17F、IL-23 和 IL-27 水平明显高于 T0 和 T1 时，且在 T2-T4 时研究牙齿的张力侧和压力侧的 IL-27 表达明显低于 T0 和 T1 时。在 T5 时，IL-17A、IL-17F、IL-23 和 IL-27 恢复到基线水平。对照组在各时间点的细胞因子均无明显差异。在 T2-T4 时，IL-17A、IL-17F 和 IL-23 的表达与 RANKL 的表达呈正相关，而 IL-27 的表达与 RANKL 的表达呈负相关。
本研究初步证实 Th17 细胞因子可能参与了 OTM 的调节。
