富白细胞血小板纤维蛋白（L-PRF）对正畸牙齿移动速度和龈沟液中各种生物标志物表达的影响。
Effect of leukocyte-platelet-rich fibrin (L-PRF) on the rate of orthodontic tooth movement and expression of various biomarkers in gingival crevicular fluid.
机构信息
Department of Dentistry, All India Institute of Medical Sciences, Sijua, Bhubaneswar, Odisha, India.
Department of Orthodontics and Dentofacial Orthopedics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
出版信息
Clin Oral Investig. 2023 May;27(5):2311-2319. doi: 10.1007/s00784-023-05026-x. Epub 2023 Apr 20.
OBJECTIVES
To assess the outcome of leukocyte-platelet-rich fibrin (L-PRF) on the rate of maxillary canine retraction and its correlation with the levels of Receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), and RANKL:OPG in the gingival crevicular fluid (GCF) during comprehensive orthodontic treatment.
SUBJECTS AND METHODS
Eighteen females who required all 1st premolars extraction for the correction of their class I bimaxillary protrusion malocclusions were included. The L-PRF plugs were placed in the experimental side 1st premolar extraction sockets. Canine retraction was performed by sliding mechanics. Canine retraction was assessed from the maxillary study models prepared just before the extraction (T) and then at 1 week (T), 2 weeks (T), 4 weeks (T), and 8 weeks (T) after the 1st premolar extraction and placement of L-PRF plugs. The concentrations of RANKL and OPG in the GCF were evaluated at T, T, T, T, and T.
RESULTS
In experimental sides, the amount of canine retraction was statistically more during the T-T, T-T, and T-T periods. The mean concentration of RANKL at T, T, and T was significantly more in the experimental sides. The mean concentration of OPG was significantly less in the experimental sides at T, T, and T. The RANKL:OPG was significantly more in the experimental sides at T, T, T, and T. No significant correlation was found between amount of canine retraction and concentration of RANKL and OPG and RANKL to OPG ratio in GCF.
CONCLUSIONS
The L-PRF accelerated the rate of maxillary canine retraction by 0.28 mm over an 8-week period. The L-PRF favored the local osteoclastogenesis by enhancing the RANKL and suppressing the OPG concentrations. There was no significant correlation between the rate of maxillary canine retraction and expression of RANKL, OPG, and RANKL:OPG in GCF.
TRIAL REGISTRATION
The Clinical Trials Registry of India (Reg. No. CTRI/2020/10/028390, Date-13.10.2020).
目的
评估富白细胞血小板纤维蛋白（L-PRF）对上颌尖牙回缩率的影响，及其与龈沟液（GCF）中核因子κB 受体激活剂配体（RANKL）、护骨素（OPG）和 RANKL：OPG 水平的相关性，在综合正畸治疗过程中。
受试者和方法
18 名女性因上颌双前突畸形需要拔除所有第一前磨牙，被纳入研究。L-PRF 塞被放置在实验侧第一前磨牙拔牙窝中。通过滑动机制进行尖牙回缩。在上颌研究模型上评估尖牙回缩，该模型在上颌第一前磨牙拔除前（T）制作，然后在 T 周、T 周、T 周、T 周和 T 周时进行评估。在 T、T、T、T 和 T 时评估 GCF 中 RANKL 和 OPG 的浓度。
结果
在实验侧，T-T、T-T 和 T-T 期间尖牙回缩量有统计学意义。T、T 和 T 时，实验侧 RANKL 的平均浓度显著更高。T、T 和 T 时，实验侧 OPG 的平均浓度显著较低。T、T、T 和 T 时，实验侧 RANKL：OPG 显著更高。未发现 GCF 中尖牙回缩量与 RANKL 和 OPG 浓度及 RANKL 与 OPG 比值之间存在显著相关性。
结论
L-PRF 在 8 周内加速了上颌尖牙回缩 0.28mm。L-PRF 通过增强 RANKL 和抑制 OPG 浓度来促进局部破骨细胞形成。GCF 中 RANKL、OPG 和 RANKL：OPG 的表达与上颌尖牙回缩率之间无显著相关性。
试验注册
印度临床试验注册处（注册号 CTRI/2020/10/028390，日期-13.10.2020）。
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