The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
J Mater Chem B. 2020 Aug 14;8(30):6469-6480. doi: 10.1039/c9tb02648g. Epub 2020 Jun 30.
Specific recognition and sensing of glycoproteins are of great importance in clinical diagnostics considering their frequent utilization as biomarkers and therapeutic targets. In this work, a biomimetic fluorescent sensor for the selective and sensitive detection of glycoprotein was developed, which was based on late-model boronate fluorescent molecularly imprinted nanoparticles (B-FMIP NPs). The B-FMIP NPs were fabricated via the macromolecular assembly of a fluorescent photo-crosslinkable amphiphilic copolymer containing boronic acid with glycoprotein in aqueous solution and in situ photo-crosslinking. Due to the synergism of boronate affinity and the molecular imprinting effect, the resultant B-FMIP NPs demonstrated specific recognition and remarkable selectivity toward the template glycoprotein (ovalbumin, OVA) with a high imprinted factor (α) of 6.0 and gave rise to obvious fluorescence quenching after binding with OVA in water. Under optimized experimental conditions, the as-prepared B-FMIP NPs exhibited linearity over the OVA concentration range of 10 to 10 mg mL with a detection limit of 3.3 × 10 mg mL, as well as a rapid response time (about 10 min), which was superior to that of other previously reported OVA sensors. Finally, these B-FMIP NPs have been applied for the determination of OVA in real samples.
基于新型硼酸盐荧光分子印迹纳米粒子（B-FMIP NPs），开发了一种用于糖蛋白选择性和灵敏检测的仿生荧光传感器。B-FMIP NPs 通过含有硼酸的荧光光交联两亲嵌段共聚物与水溶液中的糖蛋白进行大分子组装和原位光交联而制备。由于硼酸盐亲和性和分子印迹效应的协同作用，所得的 B-FMIP NPs 对模板糖蛋白（卵清蛋白，OVA）表现出特异性识别和显著的选择性，印迹因子（α）高达 6.0，并在与水合 OVA 结合后产生明显的荧光猝灭。在优化的实验条件下，所制备的 B-FMIP NPs 在 OVA 浓度范围为 10 至 10 mg mL 时表现出线性关系，检测限为 3.3×10 mg mL，以及快速的响应时间（约 10 min），优于其他先前报道的 OVA 传感器。最后，这些 B-FMIP NPs 已用于实际样品中 OVA 的测定。
