Wei Jing-Ru, Ni Yan-Li, Zhang Wei, Zhang Zhi-Qi, Zhang Jing
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, PR China.
Anal Chim Acta. 2017 Apr 1;960:110-116. doi: 10.1016/j.aca.2016.12.046. Epub 2017 Jan 5.
A novel strategy based on the fluorescent molecularly imprinted polymers (FMIP) for specific recognition and sensitive sensing glycoprotein from biological samples was developed. The FMIP prepared by introducing a fluorescent boronic acid quinoline-based on the P bead surface and assembled a glycoprotein molecularly imprinted polymer using surface imprinting technology. The resultant material showed specific recognition to model glycoprotein. At the same time, the change of fluorescence caused by the amount of model glycoprotein could achieve quantitative determination. The method provided a good linear relationship of the concentrations of HRP in the range of 0.05-1 μM, and the detection limit was 0.02 μM. It is 10 times lower than the previous fluorescence nanosensor for glycoprotein. The established method combined the desired selectivity of molecularly imprinted polymers and high sensitivity of fluorescence spectroscopy. The influence of background impurities could be effectively eliminated. The outstanding features guarantee that it can be successfully applied to detect glycoprotein from biological samples under physiological conditions.
开发了一种基于荧光分子印迹聚合物（FMIP）的新策略，用于从生物样品中特异性识别和灵敏检测糖蛋白。通过在P珠表面引入基于硼酸喹啉的荧光物质制备FMIP，并利用表面印迹技术组装糖蛋白分子印迹聚合物。所得材料对模型糖蛋白表现出特异性识别。同时，模型糖蛋白量引起的荧光变化可实现定量测定。该方法在0.05 - 1 μM范围内对HRP浓度具有良好的线性关系，检测限为0.02 μM。比先前用于糖蛋白的荧光纳米传感器低10倍。所建立的方法结合了分子印迹聚合物所需的选择性和荧光光谱的高灵敏度。可以有效消除背景杂质的影响。这些突出特点保证了它能够成功应用于在生理条件下检测生物样品中的糖蛋白。
