Cargou Marine, Ralazamahaleo Mamy, Blouin Laura, Guidicelli Gwendaline, Visentin Jonathan
CHU de Bordeaux, Laboratoire d'Immunologie et Immunogénétique, Hôpital Pellegrin, Bordeaux, France.
Université de Bordeaux, Bordeaux, France.
HLA. 2020 Sep;96(3):323-328. doi: 10.1111/tan.13986. Epub 2020 Jul 16.
PCR-sequence-specific oligonucleotide (PCR-SSO) is commonly used for HLA-typing. We recently replaced LabType SSO HD kit for HLA-C typing with the XR kit (OneLambda, Inc.). We used 137 patients' samples representing unique most likely HLA-C allele combinations to compare these kits' performance. The XR kit decreased the number of allele ambiguities with a median of 26.1% (first to third quartiles 20% to 36.2%, range 0% to 96.1%). The XR kit did not resolve all the common, intermediate and well-documented HLA allele ambiguities, which may be important in the clinical setting. The XR kit eliminated 23.6% of null allele ambiguities. In conclusion, the HLA-C XR kit provides a moderate yet valuable improvement of HLA-typing resolution in comparison with the HLA-C HD kit.
聚合酶链反应-序列特异性寡核苷酸（PCR-SSO）常用于人类白细胞抗原（HLA）分型。我们最近用XR试剂盒（OneLambda公司）取代了用于HLA-C分型的LabType SSO HD试剂盒。我们使用了137例患者样本，这些样本代表了最可能的独特HLA-C等位基因组合，以比较这两种试剂盒的性能。XR试剂盒减少了等位基因模糊性的数量，中位数为26.1%（第一至第三四分位数为20%至36.2%，范围为0%至96.1%）。XR试剂盒并未解决所有常见、中等程度以及有充分文献记载的HLA等位基因模糊性问题，而这些问题在临床环境中可能很重要。XR试剂盒消除了23.6%的无效等位基因模糊性。总之，与HLA-C HD试剂盒相比，HLA-C XR试剂盒在HLA分型分辨率方面提供了适度但有价值的改进。
