Department of Biological Sciences, Research Lab in Analytical Biochemistry and Toxicology, Midwestern Paraná State University, Guarapuava City, Paraná State, Brazil.
J Environ Sci Health B. 2020;55(9):813-819. doi: 10.1080/03601234.2020.1786327. Epub 2020 Jun 30.
The use of vegetable waste and its screening for potential cytotoxicity is of utmost importance to ensure its safe use in the feed industry for fish and other animals. We evaluated aqueous and ethanolic extracts of cooked seed coats. The Stiasny index for the aqueous and ethanolic extracts was 2.87% ± 0.03% and 60.53% ± 4.79%, respectively. Condensed tannins were 11-fold higher in the ethanolic extract than the aqueous extract. The flavonoid and polyphenol contents were 1.7- and 1.8-fold higher in the ethanolic extract than in the aqueous extract, respectively. The 36 h EC for brine shrimp hatchability was 300.32 µg/mL for the aqueous extract, and 76.60 µg/mL for the ethanolic extract. The 24 h LC was 1405.96 µg/mL for the aqueous extract, and it was 356.32 µg/mL for the ethanolic extract. The aqueous extract was nontoxic to nauplii, and therefore, it can be used as a possible food additive in fish feed. The results also demonstrated that the different solvents used in the extraction affected the yield and the total phenolic, total flavonoid, and condensed tannin content. Further and cell line cytotoxicity testing is recommended to substantiate these findings.
利用蔬菜废弃物并对其进行潜在细胞毒性筛选对于确保其在鱼类和其他动物的饲料工业中的安全使用至关重要。我们评估了煮熟的种子皮的水提物和醇提物。水提物和醇提物的 Stiasny 指数分别为 2.87%±0.03%和 60.53%±4.79%。醇提物中的缩合单宁比水提物高 11 倍。与水提物相比，醇提物中的类黄酮和多酚含量分别高 1.7 倍和 1.8 倍。盐水虾孵化率的 36 小时 EC 为水提物 300.32μg/mL，醇提物 76.60μg/mL。水提物的 24 小时 LC 为 1405.96μg/mL，醇提物为 356.32μg/mL。水提物对无节幼体无毒，因此可以用作鱼类饲料中的一种可能的食品添加剂。结果还表明，提取中使用的不同溶剂会影响产率以及总酚、总类黄酮和缩合单宁的含量。建议进一步进行细胞系细胞毒性测试来证实这些发现。
