Malencić Djordje, Maksimović Zoran, Popović Milan, Miladinović Jegor
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Bioresour Technol. 2008 Sep;99(14):6688-91. doi: 10.1016/j.biortech.2007.11.040. Epub 2007 Dec 26.
The antioxidant activity and contents of various polyphenol classes in the seeds of 20 soybean hybrids were evaluated. Total polyphenols, tannins and proanthocyanidins were determined after extraction of plant material with 70% aqueous acetone. In addition, flavonoid content was determined. Antioxidant activity of seed extracts was evaluated by DPPH free radical scavenging activity assay. A positive linear correlation between antioxidant activity and contents of total phenols, tannins and proanthocyanidins was established. The highest antioxidant activity was observed in the extracts of hybrids which have higher levels of all polyphenol classes examined. The most of the single-cross hybrids were poor in tannins which recommend them as good source for ensiled livestock feed. Results suggested that polyphenol content should be considered as an important feature of the soybean seed.
对20个大豆杂交种种子中各类多酚的抗氧化活性和含量进行了评估。用70%的丙酮水溶液提取植物材料后，测定总多酚、单宁和原花青素的含量。此外，还测定了黄酮类化合物的含量。通过DPPH自由基清除活性测定法评估种子提取物的抗氧化活性。抗氧化活性与总酚、单宁和原花青素含量之间建立了正线性关系。在所检测的所有多酚类含量较高的杂交种提取物中，观察到最高的抗氧化活性。大多数单交杂种的单宁含量较低，这表明它们是青贮家畜饲料的良好来源。结果表明，多酚含量应被视为大豆种子的一个重要特征。
