Eaton C L, Davies P, Phillips M E
Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath Park, Cardiff.
J Steroid Biochem. 1988;30(1-6):341-5. doi: 10.1016/0022-4731(88)90120-3.
The effects of EGF, TGF alpha and 5 alpha-dihydrotestosterone on the growth of a prostatic epithelial cell line have been evaluated in clonal growth assays. Similar bioassay systems have been used to identify tumour-associated growth promoters derived from a human prostatic carcinoma cell line (PC3). Growth factor activity was associated with proteins of Mr 20-30 kDa. In a separate study, EGF receptor concentration and cellular proto-oncogene expression was assessed in prostatic tumour samples. In prostatic carcinoma samples, strong correlation was observed between EGF receptor concentration and c-myc expression. There were no significant correlations between EGF receptor concentration and tumour grade or androgen receptor content in carcinoma samples. EGF receptor concentration was significantly higher in prostatic carcinoma specimens than in BPH.
在克隆生长试验中评估了表皮生长因子（EGF）、转化生长因子α（TGFα）和5α-双氢睾酮对前列腺上皮细胞系生长的影响。已使用类似的生物测定系统来鉴定源自人前列腺癌细胞系（PC3）的肿瘤相关生长促进剂。生长因子活性与分子量为20 - 30 kDa的蛋白质有关。在另一项研究中，评估了前列腺肿瘤样本中的EGF受体浓度和细胞原癌基因表达。在前列腺癌样本中，观察到EGF受体浓度与c-myc表达之间存在强相关性。在癌样本中，EGF受体浓度与肿瘤分级或雄激素受体含量之间无显著相关性。前列腺癌标本中的EGF受体浓度显著高于良性前列腺增生（BPH）。
