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鸡表皮生长因子（EGF）受体：cDNA克隆、在小鼠细胞中的表达以及EGF与转化生长因子α的差异结合

Chicken epidermal growth factor (EGF) receptor: cDNA cloning, expression in mouse cells, and differential binding of EGF and transforming growth factor alpha.

作者信息

Lax I, Johnson A, Howk R, Sap J, Bellot F, Winkler M, Ullrich A, Vennstrom B, Schlessinger J, Givol D

机构信息

Rorer Central Research, King of Prussia, Pennsylvania 19406.

出版信息

Mol Cell Biol. 1988 May;8(5):1970-8. doi: 10.1128/mcb.8.5.1970-1978.1988.

DOI:10.1128/mcb.8.5.1970-1978.1988
PMID:3260329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363375/
Abstract

The primary structure of the chicken epidermal growth factor (EGF) receptor was deduced from the sequence of a cDNA clone containing the complete coding sequence and shown to be highly homologous to the human EGF receptor. NIH-3T3 cells devoid of endogenous EGF receptor were transfected with the appropriate cDNA constructs and shown to express either chicken or human EGF receptors. Like the human EGF receptor, the chicken EGF receptor is a glycoprotein with an apparent molecular weight of 170,000. Murine EGF bound to the chicken receptor with approximately 100-fold lower affinity than to the human receptor molecule. Surprisingly, human transforming growth factor alpha (TGF-alpha) bound equally well or even better to the chicken EGF receptor than to the human EGF receptor. Moreover, TGF-alpha stimulated DNA synthesis 100-fold better than did EGF in NIH 3T3 cells that expressed the chicken EGF receptor. The differential binding and potency of mammalian EGF and TGF-alpha by the avian EGF receptor contrasts with the similar affinities of the mammalian receptor for the two growth factors.

摘要

鸡表皮生长因子（EGF）受体的一级结构是根据一个包含完整编码序列的cDNA克隆的序列推导出来的，结果表明它与人类EGF受体高度同源。用合适的cDNA构建体转染缺乏内源性EGF受体的NIH-3T3细胞，结果显示这些细胞表达鸡或人类EGF受体。与人类EGF受体一样，鸡EGF受体是一种糖蛋白，表观分子量为170,000。鼠类EGF与鸡受体的结合亲和力比与人类受体分子的结合亲和力低约100倍。令人惊讶的是，人类转化生长因子α（TGF-α）与鸡EGF受体的结合效果与人类EGF受体相当，甚至更好。此外，在表达鸡EGF受体的NIH 3T3细胞中，TGF-α刺激DNA合成的能力比EGF强100倍。禽类EGF受体对哺乳动物EGF和TGF-α的不同结合能力和效力，与哺乳动物受体对这两种生长因子的相似亲和力形成了对比。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/363375/01a3cadeba41/molcellb00065-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/363375/313891c86277/molcellb00065-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/363375/01a3cadeba41/molcellb00065-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/363375/313891c86277/molcellb00065-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/363375/01a3cadeba41/molcellb00065-0125-a.jpg

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