Yamamoto T, Nishida T, Miyajima N, Kawai S, Ooi T, Toyoshima K
Cell. 1983 Nov;35(1):71-8. doi: 10.1016/0092-8674(83)90209-x.
The erbB gene of an avian erythroblastosis virus, AEV-H, was determined to be 1812 nucleotides long and was predicted to code for a protein of 67,638 daltons. Unexpectedly, a sequence of 285 amino acids in the middle of the protein showed a significant homology (38%) with the sequence in the carboxy terminus of p60src. The nucleotide sequence of a mutant of AEV-H, td-130, which induces sarcomas but not erythroblastosis in chicken, was also analyzed. A deletion of 169 nucleotides was identified in the 3' half of the erbB gene, indicating that the gene codes for a truncated protein with the predicted molecular weight of 46,667. These findings suggest that the homologous domain of erbB protein with its N-terminal portion is sufficient for the transformation of fibroblasts and that one-third of the carboxy-terminal domain has a key role for the transformation of erythroid cells.
禽成红细胞增多症病毒AEV-H的erbB基因经测定长度为1812个核苷酸，预计编码一种分子量为67,638道尔顿的蛋白质。出乎意料的是，该蛋白质中间一段285个氨基酸的序列与p60src羧基末端的序列显示出显著的同源性（38%）。还分析了AEV-H的一个突变体td-130的核苷酸序列，该突变体在鸡中诱导肉瘤但不诱导成红细胞增多症。在erbB基因的3'端一半区域发现了169个核苷酸的缺失，这表明该基因编码一种预计分子量为46,667的截短蛋白。这些发现表明，erbB蛋白与其N端部分的同源结构域足以使成纤维细胞发生转化，而羧基末端结构域的三分之一对红系细胞的转化起关键作用。
