Microbiological Laboratory, Huzhou Center for Disease Control and Prevention, Huzhou, China.
Obstetrics and Gynecology, Huzhou Maternity and Child Health Care Hospital, Huzhou, China.
J Med Virol. 2020 Dec;92(12):3173-3178. doi: 10.1002/jmv.26247. Epub 2020 Aug 25.
Norovirus is an important causative agent of acute gastroenteritis worldwide, affecting people of all ages. Stool samples collected from patients with clinical symptoms of acute gastroenteritis in all age groups at the diarrhea outpatient department of the First People's Hospital in Huzhou were analyzed to gain insight into the prevalence and genetic characteristics of norovirus. From January to December 2018, a total of 551 specimens were screened for norovirus by real-time reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR was used for genomic amplification and sequencing of the RNA-dependent RNA polymerase and capsid gene of the positive samples. Genotypes of norovirus were assigned using the norovirus Noronet typing tool and phylogenetic analysis. About 100 (18.1%) specimens were identified as norovirus positive. GII genogroup was the main genogroup identified (83.0%; 83/100). About 42 (42.0%) samples were successfully sequenced and genotyped by RT-PCR. Since one of the samples was dual infection, so we got 43 virus finally. Nine norovirus GII genotypes and four norovirus GI genotypes were detected in Huzhou during our research period. The main two norovirus GII genotypes were GII.2[P16] (54.8%; 23/43) and GII.17[P17] (11.9%; 5/43). We characterized the molecular epidemiology of norovirus infection in acute gastroenteritis patients during 2018. GII genogroup was the main genogroup identified. The dominance norovirus genotype circulating in the population of Huzhou was GII.2[P16] in 2018.
诺如病毒是全球范围内引起急性胃肠炎的重要病原体，影响所有年龄段的人群。本研究分析了湖州市第一人民医院腹泻门诊各年龄段具有临床急性胃肠炎症状患者的粪便标本，旨在了解诺如病毒的流行情况和遗传特征。2018 年 1 月至 12 月，采用实时逆转录-聚合酶链反应（RT-PCR）对 551 份标本进行诺如病毒筛查。对阳性样本的 RNA 依赖性 RNA 聚合酶和衣壳基因进行 RT-PCR 基因组扩增和测序。利用诺如病毒 Noronet 分型工具和系统进化分析对诺如病毒基因型进行赋值。约 100 份（18.1%）标本鉴定为诺如病毒阳性。GII 基因型是主要鉴定的基因型（83.0%；83/100）。约 42 份（42.0%）样本通过 RT-PCR 成功测序和分型。由于其中一个样本为双重感染，因此我们最终得到了 43 株病毒。在研究期间，在湖州共检测到 9 种诺如病毒 GII 基因型和 4 种诺如病毒 GI 基因型。主要的两种诺如病毒 GII 基因型为 GII.2[P16]（54.8%；23/43）和 GII.17[P17]（11.9%；5/43）。我们对 2018 年急性胃肠炎患者的诺如病毒感染分子流行病学特征进行了分析。鉴定到的主要基因型为 GII 基因型。2018 年，湖州市流行的优势诺如病毒基因型为 GII.2[P16]。
