National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, China.
National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, China.
Microbiol Res. 2020 Sep;238:126531. doi: 10.1016/j.micres.2020.126531. Epub 2020 Jun 24.
Phytotoxins are widely found in plant pathogens. In recent years, many diseases caused by Neopestalotiopsis clavispora have been reported. To better understand the pathogenicity of N. clavispora, a solid fermentation strategy was employed to isolate and identify virulence factors afritoxinone B, afritoxinone A and oxysporone. The phytotoxic activities of these toxins were evaluated. Oxysporone exhibited high levels of phytotoxic activity after 72 h and the lesion area ranged from 21.5-84.3 mm2 after 9 days of treatment. The phytotoxic activities of the other two compounds were lower than that for oxysporone. The phytotoxic activity towards non-host organisms was also assessed for the three analyzed compounds; phytotoxic activity was observed in each case. Based on these results, we conclude that oxysporone is the main virulence factor in N. clavispora. We also suggest that each of the three compounds were non-host-specific toxins (NHST). To our knowledge, this is the first study to analyze phytotoxins produced by N. clavispora.
植物毒素广泛存在于植物病原体中。近年来，报道了许多由 Neopestalotiopsis clavispora 引起的疾病。为了更好地了解 N. clavispora 的致病性，采用固体发酵策略分离和鉴定了致病因子 afritoxinone B、afritoxinone A 和 oxysporone。评估了这些毒素的植物毒性活性。Oxysporone 在 72 小时后表现出高植物毒性活性，在处理 9 天后的损伤面积范围为 21.5-84.3 mm2。另外两种化合物的植物毒性活性低于 oxysporone。还评估了三种分析化合物对非宿主生物的植物毒性活性；在每种情况下都观察到了植物毒性活性。基于这些结果，我们得出结论，oxysporone 是 N. clavispora 中的主要致病因子。我们还建议，这三种化合物均为非宿主特异性毒素（NHST）。据我们所知，这是首次分析 N. clavispora 产生的植物毒素。
