CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, CAS Engineering Laboratory for Kiwifruit Industrial Technology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
College of Life Sciences, Jiamusi University, Jiamusi, 154007, China.
BMC Genomics. 2024 Sep 6;25(1):839. doi: 10.1186/s12864-024-10751-y.
The postharvest rot of kiwifruit is one of the most devastating diseases affecting kiwifruit quality worldwide. However, the genomic basis and pathogenicity mechanisms of kiwifruit rot pathogens are lacking. Here we report the first whole genome sequence of Pestalotiopsis microspora, one of the main pathogens causing postharvest kiwifruit rot in China. The genome of strain KFRD-2 was sequenced, de novo assembled, and analyzed.
The genome of KFRD-2 was estimated to be approximately 50.31 Mb in size, with an overall GC content of 50.25%. Among 14,711 predicted genes, 14,423 (98.04%) exhibited significant matches to genes in the NCBI nr database. A phylogenetic analysis of 26 known pathogenic fungi, including P. microspora KFRD-2, based on conserved orthologous genes, revealed that KFRD-2's closest evolutionary relationships were to Neopestalotiopsis spp. Among KFRD-2's coding genes, 870 putative CAZy genes spanned six classes of CAZys, which play roles in degrading plant cell walls. Out of the 25 other plant pathogenic fungi, P. microspora possessed a greater number of CAZy genes than 22 and was especially enriched in GH and AA genes. A total of 845 transcription factors and 86 secondary metabolism gene clusters were predicted, representing various types. Furthermore, 28 effectors and 109 virulence-enhanced factors were identified using the PHI (pathogen host-interacting) database.
This complete genome sequence analysis of the kiwifruit postharvest rot pathogen P. microspora enriches our understanding its disease pathogenesis and virulence. This study establishes a theoretical foundation for future investigations into the pathogenic mechanisms of P. microspora and the development of enhanced strategies for the efficient management of kiwifruit postharvest rots.
猕猴桃采后腐烂是全球范围内影响猕猴桃品质最严重的病害之一。然而，猕猴桃腐烂病原菌的基因组基础和致病性机制仍不清楚。本研究报道了中国猕猴桃采后腐烂主要病原菌之一拟盘多毛孢（Pestalotiopsis microspora）的首个全基因组序列。对 KFRD-2 菌株进行了测序、从头组装和分析。
KFRD-2 的基因组大小约为 50.31 Mb，GC 含量为 50.25%。在预测的 14711 个基因中，有 14423 个（98.04%）与 NCBI nr 数据库中的基因有显著匹配。基于保守直系同源基因对包括 P. microspora KFRD-2 在内的 26 种已知致病性真菌进行的系统发育分析表明，KFRD-2 与 Neopestalotiopsis spp 的进化关系最为密切。在 KFRD-2 的编码基因中，870 个假定的 CAZy 基因跨越 CAZys 的六个类别，这些基因在降解植物细胞壁中发挥作用。在其他 25 种植物病原真菌中，P. microspora 拥有比 22 种更多的 CAZy 基因，尤其是在 GH 和 AA 基因方面。共预测到 845 个转录因子和 86 个次级代谢基因簇，代表了多种类型。此外，使用 PHI（病原体-宿主相互作用）数据库鉴定了 28 个效应子和 109 个增强毒力的因子。
本研究对猕猴桃采后腐烂病原菌 P. microspora 的全基因组序列分析丰富了我们对其致病机制和毒力的认识。本研究为进一步研究 P. microspora 的致病机制以及开发高效的猕猴桃采后腐烂防治策略奠定了理论基础。
