Hofmann G E, Rao C V, Carman F R, Siddiqi T A
Department of Obstetrics and Gynecology, University of Cincinnati School of Medicine, OH.
Acta Endocrinol (Copenh). 1988 Apr;117(4):485-90. doi: 10.1530/acta.0.1170485.
Specific binding of 125I-human epidermal growth factor (hEGF) to homogenates of term human placentas and fetal membranes from normal and appropriate for gestational age (N = 20), intrauterine growth retarded (N = 9), twin (N = 11), White class A/B diabetic (N = 12), and large for gestational age (N = 13) pregnancies was measured. In all pregnancy states, placentas bound approximately four times more 125I-hEGF than did fetal membranes (P less than 0.001). There was no significant difference in 125I-hEGF binding to fetal membranes from the various pregnancy states (P greater than 0.05). 125I-hEGF specific binding to placentas from intrauterine growth retarded or twin pregnancies was significantly greater compared with placentas from normal and appropriate for gestational age pregnancies (P less than 0.05). The binding to placentas from pregnancies complicated by White class A/B diabetes or large for gestational age infants, on the other hand, was not significantly different from that to placentas from normal and appropriate for gestational age pregnancies. 125I-hEGF specific binding did not differ between placentas from intrauterine growth retarded or twin pregnancies (P greater than 0.05). Placental and fetal membrane 125I-hEGF binding did not vary with fetal sex, maternal race, placental weight, or gestational age between 37 to 42 weeks (P greater than 0.05). Placental but not fetal membrane 125I-hEGF binding increased with increasing infant weight when appropriate for gestational age and large for gestational age infants were included (P less than 0.05, r = 0.38, N = 32) but not for intrauterine growth retarded, appropriate for gestational age, or large for gestational age infants alone.
测定了¹²⁵I-人表皮生长因子（hEGF）与足月正常且孕周合适（N = 20）、宫内生长受限（N = 9）、双胎（N = 11）、白种人A/B级糖尿病（N = 12）及大于孕周（N = 13）妊娠的人胎盘和胎膜匀浆的特异性结合。在所有妊娠状态下，胎盘结合的¹²⁵I-hEGF约为胎膜的四倍（P < 0.001）。不同妊娠状态下胎膜对¹²⁵I-hEGF的结合无显著差异（P > 0.05）。与正常且孕周合适妊娠的胎盘相比，¹²⁵I-hEGF与宫内生长受限或双胎妊娠胎盘的特异性结合显著增加（P < 0.05）。另一方面，与正常且孕周合适妊娠的胎盘相比，¹²⁵I-hEGF与合并白种人A/B级糖尿病或大于孕周婴儿妊娠的胎盘的结合无显著差异。¹²⁵I-hEGF与宫内生长受限或双胎妊娠胎盘的特异性结合无差异（P > 0.05）。胎盘和胎膜的¹²⁵I-hEGF结合在胎儿性别、母亲种族、胎盘重量或37至42周的孕周之间无变化（P > 0.05）。当纳入孕周合适和大于孕周的婴儿时，胎盘而非胎膜的¹²⁵I-hEGF结合随婴儿体重增加而增加（P < 0.05，r = 0.38，N = 32），但单独针对宫内生长受限、孕周合适或大于孕周的婴儿则不然。
