Laidoudi Younes, Medkour Hacène, Latrofa Maria Stefania, Davoust Bernard, Diatta Georges, Sokhna Cheikh, Barciela Amanda, Hernandez-Aguilar R Adriana, Raoult Didier, Otranto Domenico, Mediannikov Oleg
IRD, AP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, France.
IHU Méditerranée Infection, 19-21, Bd Jean Moulin, 13005 Marseille, France.
Pathogens. 2020 Jun 27;9(7):517. doi: 10.3390/pathogens9070517.
(syn. ) has been reported in human and various non-human primates including great apes. The identification of this nematode is seldom performed and relies on egg characterization at the coproscopy, in the absence of any molecular tool. Following the recovery of two adult females of from the feces of wild Senegalese chimpanzees, morphometric characteristics were reported and new data on the width of the esophagus (0.268-0.287 mm) and on the cuticle structure (0.70-0.122 mm) were provided. The molecular characterization of a set of mitochondrial (1, 16S rRNA, 12S rRNA) and nuclear (18S rRNA and ITS2) partial genes was performed. Our phylogenetic analysis indicates for the first time that is monophyletic with species. A novel molecular tool was developed for the routine diagnosis of and the surveillance of Nematoda infestations. An -specific qPCR targeting the 12S gene was assessed. The assay was able to detect up to 1.13 × 10 eggs/g of fecal matter irrespective of its consistency, with an efficiency of 101.8% and a perfect adjustment (R = 0.99). The infection rate by in the chimpanzee fecal samples was 52.08%. Only 6.19% of the environmental samples were positive for nematode DNA and any for . Our findings indicate the need for further studies to clarify the epidemiology, circulation, life cycle, and possible pathological effects of this infestation using the molecular tool herein developed.
（同义词）已在人类和包括大猩猩在内的各种非人类灵长类动物中被报道。在没有任何分子工具的情况下，这种线虫的鉴定很少进行，且依赖于粪便检查时的虫卵特征。从野生塞内加尔黑猩猩的粪便中发现两只成年雌性线虫后，报告了其形态特征，并提供了关于食管宽度（0.268 - 0.287毫米）和角质层结构（0.70 - 0.122毫米）的新数据。对一组线粒体（细胞色素c氧化酶亚基I、16S rRNA、12S rRNA）和核（18S rRNA和ITS2）部分基因进行了分子特征分析。我们的系统发育分析首次表明，[线虫名称]与[相关线虫名称]物种是单系的。开发了一种用于[线虫名称]常规诊断和线虫感染监测的新型分子工具。评估了一种针对12S基因的[线虫名称]特异性qPCR。该检测方法能够检测到每克粪便中多达1.13×10个虫卵，无论粪便的稠度如何，效率为101.8%，拟合度良好（R = 0.99）。黑猩猩粪便样本中[线虫名称]的感染率为52.08%。仅6.19%的环境样本线虫DNA呈阳性，且没有样本对[线虫名称]呈阳性。我们的研究结果表明，需要进一步研究以利用本文开发的分子工具阐明这种感染的流行病学、传播、生命周期以及可能的病理影响。
