National and Kapodistrian University of Athens, School of Health Sciences, Department of Pharmacy, Athens, Greece.
National and Kapodistrian University of Athens, School of Medicine, Department of Experimental Physiology, Athens, Greece.
In Vivo. 2020 Jul-Aug;34(4):1835-1843. doi: 10.21873/invivo.11978.
BACKGROUND/AIM: Cigarette smoke (CS) is a major environmental health threat. The oxidative stress induced by CS on keratinocytes and the possible protective effect of nicotine, its receptor inhibitors, and Pinus halepensis bark extract in relation to known antioxidants were investigated.
Primary mouse keratinocytes were exposed to cigarette smoke in the presence and absence of Pinus halepensis bark extract (1 μg/ml), rutin (50 μM) and ascorbic acid (250 μM), nicotine (1 μM) with or without mecamylamine (5 μM) and α-bungarotoxin (0.1 μM). Keratinocyte viability and oxidative stress were evaluated by MTT and fluorescence assays.
Pinus halepensis bark extract decreased the oxidative stress and increased the viability of keratinocytes, and moreover, these effects were more pronounced compared to the mixture of rutin and L-ascorbic acid. Nicotine significantly enhanced the viability potentiation of the beneficial effect induced by Pinus halepensis bark extract. Mecamylamine and α-bungarotoxin showed no specific effect.
Pinus halepensis bark extract in combination with nicotine may successfully reverse skin damage induced by cigarette smoke.
背景/目的：香烟烟雾（CS）是主要的环境健康威胁。本研究旨在调查 CS 对角质形成细胞造成的氧化应激，以及尼古丁、其受体抑制剂和欧洲赤松皮提取物对已知抗氧化剂的可能保护作用。
将原代小鼠角质形成细胞暴露于香烟烟雾中，同时加入或不加入欧洲赤松皮提取物（1μg/ml）、芦丁（50μM）和抗坏血酸（250μM）、尼古丁（1μM），以及美加仑胺（5μM）和α-银环蛇毒素（0.1μM）。通过 MTT 和荧光测定评估角质形成细胞活力和氧化应激。
欧洲赤松皮提取物降低了角质形成细胞的氧化应激并增加了其活力，并且与芦丁和 L-抗坏血酸的混合物相比，这些作用更为显著。尼古丁显著增强了欧洲赤松皮提取物诱导的有益作用的活力增强作用。美加仑胺和α-银环蛇毒素没有显示出特定的作用。
欧洲赤松皮提取物与尼古丁联合使用可能成功逆转香烟烟雾引起的皮肤损伤。
