Zhang Yu, Tang Yang, Tan Congrui, Xu Wei
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
ACS Cent Sci. 2020 Jun 24;6(6):1001-1008. doi: 10.1021/acscentsci.0c00622. Epub 2020 Jun 5.
The shape and structure analyses capability of nanopore is powerful and complementary to mass spectrometry analysis. It is extremely attractive but challenging to integrate these two techniques. The feasibility of combining nanopore electrospray with mass spectrometry was explored in this study. A nanopore effect was observed during the nano-electrospray of single bacterium, through which the shape and dimension of a single bacterium could be obtained. Molecular information on these bacteria was then acquired by analyzing these bacteria deposited on the counter electrode through laser spray ionization mass spectrometry experiments. Proof-of-concept experiments were carried out for four types of bacteria. Results show that the combination of nanopore results with mass spectrum data could effectively improve the identification accuracy of these bacteria from 72.5% to 100%. Although initial experiments were demonstrated in this work, results showed that it is feasible and promising to integrate nanopore technology with mass spectrometry for large biomolecule studies in the near future.
纳米孔的形状和结构分析能力很强，与质谱分析具有互补性。将这两种技术整合极具吸引力，但也具有挑战性。本研究探讨了将纳米孔电喷雾与质谱联用的可行性。在单个细菌的纳米电喷雾过程中观察到了纳米孔效应，通过该效应可以获得单个细菌的形状和尺寸。然后通过激光喷雾电离质谱实验分析沉积在反电极上的这些细菌，从而获取有关这些细菌的分子信息。针对四种类型的细菌进行了概念验证实验。结果表明，将纳米孔结果与质谱数据相结合可以有效地将这些细菌的识别准确率从72.5%提高到100%。尽管本工作展示的是初步实验，但结果表明在不久的将来将纳米孔技术与质谱联用用于大型生物分子研究是可行且有前景的。
