流式细胞术分选肝脏巨噬细胞。

Liver Macrophage Isolation by Flow Cytometry Sorting.

机构信息

Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden.

出版信息

Methods Mol Biol. 2020;2164:15-20. doi: 10.1007/978-1-0716-0704-6_3.

Abstract

Liver macrophages are immune cells that under steady state conditions play important roles in maintaining tissue homeostasis. But these cells are also intricately linked to the pathogenesis of obesity-related diseases such as nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and type 2 diabetes (T2D). Described herein is a protocol to isolate liver macrophages by FACS for downstream molecular analysis. Such studies of macrophages are important for elucidating their role in the pathogenesis of these metabolic diseases.

摘要

肝巨噬细胞是免疫细胞，在稳态条件下，它们在维持组织内稳态方面发挥着重要作用。但这些细胞也与肥胖相关疾病的发病机制密切相关，如非酒精性脂肪性肝病 (NAFLD)、非酒精性脂肪性肝炎 (NASH) 和 2 型糖尿病 (T2D)。本文描述了一种通过 FACS 分离肝巨噬细胞进行下游分子分析的方案。对巨噬细胞的这些研究对于阐明它们在这些代谢性疾病发病机制中的作用非常重要。