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通过流式细胞术和免疫荧光技术对 NASH 小鼠肝脏巨噬细胞组成进行全面分析。

Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH.

机构信息

Diabetes Research Center, Washington University School of Medicine, St. Louis, MO 63110, USA.

Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.

出版信息

STAR Protoc. 2021 Apr 29;2(2):100511. doi: 10.1016/j.xpro.2021.100511. eCollection 2021 Jun 18.

DOI:10.1016/j.xpro.2021.100511
PMID:33997821
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8102804/
Abstract

Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

摘要

最近，人们已经清楚地认识到，在非酒精性脂肪性肝炎（NASH）的发病机制中，肝脏中的巨噬细胞多样性增加。在这里，我们提供了一种详细的方案，用于使用流式细胞术和免疫荧光（IF）分析非酒精性脂肪肝（NAFLD）和早期 NASH 小鼠的肝脏巨噬细胞亚群。这些方法可用于评估 NASH 期间巨噬细胞亚群的组成和定位。有关此方案的使用和执行的完整详细信息，请参阅 Daemen 等人（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/2566a4115bdc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/815745a801c0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/ab7ace94d44c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/2566a4115bdc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/815745a801c0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/ab7ace94d44c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb2e/8102804/2566a4115bdc/gr2.jpg

相似文献

[1]
Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH.

STAR Protoc. 2021-6-18

[2]
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[3]
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[4]
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[5]
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[6]
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[7]
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[8]
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[9]
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[10]
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[10]
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本文引用的文献

[1]
Dynamic Shifts in the Composition of Resident and Recruited Macrophages Influence Tissue Remodeling in NASH.

Cell Rep. 2021-1-12

[2]
Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.

Immunity. 2020-9-15

[3]
Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis.

Immunity. 2020-9-15

[4]
Niche-Specific Reprogramming of Epigenetic Landscapes Drives Myeloid Cell Diversity in Nonalcoholic Steatohepatitis.

Immunity. 2020-6-16

[5]
Landscape of Intercellular Crosstalk in Healthy and NASH Liver Revealed by Single-Cell Secretome Gene Analysis.

Mol Cell. 2019-8-8

[6]
An efficient method to isolate Kupffer cells eliminating endothelial cell contamination and selective bias.

J Leukoc Biol. 2018-4-1

[7]
Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells.

Nat Commun. 2016-1-27

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