叶片和莲座测量抗冻性：电解质渗漏和叶绿素荧光测定。

Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays.

机构信息

Physikalische Biochemie, Universität Potsdam, Potsdam, Germany.

Department of Chemistry and Bioscience, Aalborg University, Aalborg East, Denmark.

出版信息

Methods Mol Biol. 2020;2156:9-21. doi: 10.1007/978-1-0716-0660-5_2.

DOI:10.1007/978-1-0716-0660-5_2

PMID:32607971

Abstract

Quantitative assessment of freezing tolerance is essential to unravel plant adaptations to cold temperatures. Not only the survival of whole plants, but also impairment of detached leaves or small rosettes after a freeze-thaw cycle can be used to accurately quantify plant freezing tolerance in terms of LT values. Here we describe two methods to determine the freezing tolerance of detached leaves or rosettes using a full or selected set of freezing temperatures and an additional method using chlorophyll fluorescence as a different physiological parameter. Firstly, we illustrate how to assess the integrity of (predominantly) the plasma membrane during freezing using an electrolyte leakage assay. Secondly, we provide a chlorophyll fluorescence imaging protocol to determine the freezing tolerance of the photosynthetic apparatus.

摘要

定量评估植物的抗寒性对于揭示植物适应低温环境的机制至关重要。不仅可以使用整个植物的存活率，还可以使用经过冻融循环后的离体叶片或小莲座叶盘的损伤程度，来准确地以 LT 值来量化植物的抗寒性。在这里，我们描述了两种使用全或部分冷冻温度来确定离体叶片或莲座叶盘抗寒性的方法，以及一种使用叶绿素荧光作为不同生理参数的方法。首先，我们将展示如何使用电解质渗漏测定法评估（主要是）质膜在冷冻过程中的完整性。其次，我们提供了叶绿素荧光成像方案来确定光合作用机构的抗寒性。