Kameniarová Michaela, Černý Martin, Novák Jan, Ondrisková Vladěna, Hrušková Lenka, Berka Miroslav, Vankova Radomira, Brzobohatý Bretislav
Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, Brno, Czechia.
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, The Czech Academy of Sciences, Prague, Czechia.
Front Plant Sci. 2022 Jun 9;13:887103. doi: 10.3389/fpls.2022.887103. eCollection 2022.
The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in 1-month-old (Col-0) plants exposed for 1 week to 4°C at short-day conditions under white (100 and 20 μmol ms), blue, or red (20 μmol ms) light conditions. An upregulated expression of , inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in accessions originating from different and contrasting latitudes. In all but one accession, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light-treated plants at low temperatures and showed that the cold response is highly accession-specific. In general, blue light increased mainly the cold-stress-related proteins and red light-induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light-driven cell function maintaining program and blue light-activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.
冷驯化过程受许多因素调节，如环境温度、日照长度、光照强度或激素状态。对在不同光质条件下生长的植物进行的实验表明，植物对低温的反应也是一个依赖光质的过程。在此，研究了光质在冷反应中的作用，实验选用1月龄（Col-0）植株，在短日照条件下，分别置于白光（100和20 μmol·m⁻²·s⁻¹）、蓝光或红光（20 μmol·m⁻²·s⁻¹）下，于4°C处理1周。[具体基因名称]表达上调、光合作用受到抑制以及膜损伤增加，表明蓝光增强了低温的影响。有趣的是，与白光低温处理的植株相比，蓝光和红光处理的冷处理植株仅表现出有限的抗冻性。接下来，在来自不同且对比鲜明的纬度的[具体植物名称]种质中评估了冷反应中光质信号的特异性。除了一个[具体植物名称]种质外，在所有种质中，蓝光均增加了低温对光合参数和电解质渗漏的影响。对于缺乏功能性CRY2蛋白的Ws-0种质未发现这种效应，这表明CRY2蛋白在冷反应中发挥作用。蛋白质组学数据证实了低温下红光和蓝光处理植株之间存在显著差异，并表明冷反应具有高度的种质特异性。总体而言，蓝光主要增加了与冷胁迫相关的蛋白质，而红光诱导叶绿体相关蛋白质的表达更高，这与红光冷处理植株中较高的光合参数相关。总之，我们的数据表明，光在冷处理过程中调节两种不同的机制——红光驱动的细胞功能维持程序和蓝光激活的特异性冷反应。白光下冷处理植株具有显著更高的抗冻性，证明了这些机制相互补充的重要性。
