Department of Plant Biotechnology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany.
Plant Evolutionary Cell Biology, Department Biology I, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.
Methods Mol Biol. 2020;2156:269-275. doi: 10.1007/978-1-0716-0660-5_18.
Plant cells are heavily compartmentalized, and metabolite concentrations in the various compartments differ significantly. Thus, determination of metabolite abundance in whole-cell extracts may be misleading, when the role of a compound in plant freezing tolerance shall be evaluated. Here, we describe a method for the separation of the largest compartments of plant cells, the vacuole, plastid, and cytosol. With more elaborate analysis, this method can be expanded to also resolve mitochondria and other compartments.
植物细胞高度区隔化，不同隔室内的代谢物浓度有显著差异。因此，当评估化合物在植物抗冻性中的作用时，仅从全细胞提取物中测定代谢物丰度可能会产生误导。在此，我们描述了一种分离植物细胞最大隔室（液泡、质体和细胞质）的方法。通过更精细的分析，此方法还可以扩展到分离线粒体和其他隔室。
