Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, India.
Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
Nat Prod Res. 2021 Dec;35(23):5489-5492. doi: 10.1080/14786419.2020.1786830. Epub 2020 Jul 1.
A HP20 resin-based unique method was adopted to get an active fraction of the hydroalcoholic extract of roots. The fraction showed potent cytotoxicity against cancer cell line and was further subjected to detailed phytochemical investigation to obtain ten biomarkers. The isolated compounds were also tested for the cytotoxicity against the C6 glioma cell line using MTT assay. Among the isolated compounds, glycyrrhetic acid (), glabrol (), and glabridin () exhibited significant cytotoxicity. The compounds showed a dose-dependent decrease in cell viability. The active compounds were subjected to molecular docking study against topoisomerase I and topoisomerase II to support the mechanism of antitumor activity.
采用 HP20 树脂基独特方法从 根的水醇提取物中获得活性部分。该部分对癌细胞系表现出强烈的细胞毒性，并进一步进行详细的植物化学研究以获得十种生物标志物。用 MTT 测定法还测试了分离的化合物对 C6 神经胶质瘤细胞系的细胞毒性。在所分离的化合物中，甘草次酸（）、光甘草定（）和甘草素（）表现出显著的细胞毒性。这些化合物表现出剂量依赖性的细胞活力下降。将活性化合物进行拓扑异构酶 I 和拓扑异构酶 II 的分子对接研究，以支持抗肿瘤活性的机制。
