穿心莲和甘草对口腔癌细胞系的抗癌作用：一项体外实验研究。
Anticancer Effect of Plectranthus Amboinicus and Glycyrrhiza Glabra on Oral Cancer Cell Line: An Invitro Experimental Study.
机构信息
Chettinad Dental College and Research Institute (CDCRI), Kelambakkam, Chennai, India.
出版信息
Asian Pac J Cancer Prev. 2023 Mar 1;24(3):881-887. doi: 10.31557/APJCP.2023.24.3.881.
BACKGROUND AND OBJECTIVE
Oral cancer is a commonly encountered type of cancer with multifactorial etiology. The number of oral cancer cases are increasing in the recent past. Advancements in cancer therapy are continuously evolving. In spite of that, the survival rate is very low along with adverse effects associated with each treatment modality. This poses a need for a constant search for newer or alternative treatment options. Herbal medicines or plant-based medicines are derived from plant sources. Evidence supports that incorporating herbal medicines in cancer therapy offers many advantages. Hence, exploring plant species for anticarcinogenic properties can potentially benefit cancer therapy. Studies show that the two medicinal herbs Plectranthus amboinicus (Indian borage, Karpooravalli) and Glycyrrhiza glabra (Liquorice, Athimathuram) have shown excellent anticancer activity on various cancers. The present study aimed to evaluate and assess the anticancer property of two medicinal plants Plectranthus amboinicus (Indian borage, Karpooravalli) and Glycyrrhiza glabra (Liquorice, Athimathuram) on oral cancer (KB) cell line.
MATERIALS AND METHODS
Ethanolic extracts of leaves of Plectranthus amboinicus and roots of Glycyrrhiza glabra were prepared. The oral cancer (KB) cell line was procured and cultured. Cell viability (MTT) assay was performed using various concentrations of both the plant extracts on oral cancer cells. The percentage of cell viability for each concentration was calculated and the IC50 value was derived for the two plant extracts.
RESULTS
The results revealed a significant decrease in the percentage of viable cells with increasing concentration of the extracts. The IC50 values of Plectranthus amboinicus and Glycyrrhiza glabra were 53.0 µg/ml and 43.6 µg/ml respectively. On comparing the anticancer effect of the two extracts, Glycyrrhiza glabra was more cytotoxic than Plectranthus amboinicus on oral cancer (KB) cells.
CONCLUSION
The two medicinal plants Plectranthus amboinicus and Glycyrrhiza glabra exhibited potent anticancer activity against oral cancer (KB) cells.
背景与目的
口腔癌是一种常见的癌症类型，具有多因素病因。在最近的过去，口腔癌的病例数量一直在增加。癌症治疗的进展不断发展。尽管如此，生存率仍然很低，并且每种治疗方式都伴随着不良反应。这就需要不断寻找新的或替代的治疗选择。草药或植物药来源于植物来源。有证据表明，将草药纳入癌症治疗中具有许多优势。因此，探索具有抗癌特性的植物物种可能有益于癌症治疗。研究表明，两种药用植物 Plectranthus amboinicus（印度琉璃苣，Karpooravalli）和 Glycyrrhiza glabra（甘草，Athimathuram）对各种癌症均表现出出色的抗癌活性。本研究旨在评估两种药用植物 Plectranthus amboinicus（印度琉璃苣，Karpooravalli）和 Glycyrrhiza glabra（甘草，Athimathuram）对口腔癌（KB）细胞系的抗癌特性。
材料与方法
制备了 Plectranthus amboinicus 叶和 Glycyrrhiza glabra 根的乙醇提取物。获得并培养口腔癌（KB）细胞系。使用两种植物提取物的不同浓度在口腔癌细胞上进行细胞活力（MTT）测定。计算每个浓度的细胞活力百分比，并得出两种植物提取物的 IC50 值。
结果
结果表明，随着提取物浓度的增加，存活细胞的百分比显着降低。Plectranthus amboinicus 和 Glycyrrhiza glabra 的 IC50 值分别为 53.0 µg/ml 和 43.6 µg/ml。比较两种提取物的抗癌作用，甘草对口腔癌（KB）细胞的细胞毒性强于印度琉璃苣。
结论
两种药用植物 Plectranthus amboinicus 和 Glycyrrhiza glabra 对口腔癌（KB）细胞表现出强大的抗癌活性。
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