Sha Yun-Fei, Sun Xing-Bin, Xin Wen-Peng, Gao Hao-Ze, Cheng Shou-Tao, Gao Min, Wang Xu-Ming
College of Forestry, Northeast Forestry University, Harbin 150040, China.
Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Huan Jing Ke Xue. 2020 Feb 8;41(2):656-664. doi: 10.13227/j.hjkx.201907049.
Confined animal feeding operations generate high concentrations of airborne antibiotic-resistant bacteria, including pathogenic strains that may pose a health risk to both animals and farm workers and pollute the local air environment. In this study, tetracycline and erythromycin-resistant bacteria were used as examples to study the biodiversity and community structure of airborne antibiotic-resistant bacteria in animal farms. The Anderson sampler was used to collect bioaerosols samples from the inside environment and outside atmospheric environments. A comparative analysis of biological differences of antibiotic-resistant bacteria was conducted on fine and coarse particles, bioaerosol samples inside the house, fecal samples, and inside and outside bioaerosol samples based on the result of the Illumina MiSeq sequencing. The key genus that drives the above differences was also studied. Results showed that the biodiversity of airborne erythromycin-resistant bacteria was higher than that of airborne tetracycline-resistant bacteria, and the biodiversity of bioaerosol samples in the house was higher than that in fecal samples. There were no significant differences in the biodiversity and community structure of airborne antibiotic-resistant bacteria between fine and coarse particles. Actinobacteria is one of the key bacteria responsible for the differences between erythromycin-resistant bacteria and other bacterial populations. is one of the key genera of tetracycline-resistant flora that is distinguished from erythromycin resistance and all bacterial flora. The results of the community structure showed that there was no significant difference in the dominant flora and the community structure of tetracycline and erythromycin-resistant bacteria. The community structure of feces and bioaerosol samples is different at the genus level, and the dominant bacteria are likewise different. The results of this study provide basic data for the accurate assessment of the current status of antibiotic-resistant bacteria in animal farms and their ecological risks.
集约化动物饲养场会产生高浓度的空气传播抗生素抗性细菌，包括可能对动物和农场工人健康构成风险并污染当地空气环境的致病菌株。在本研究中，以四环素和红霉素抗性细菌为例，研究动物养殖场空气传播抗生素抗性细菌的生物多样性和群落结构。使用安德森采样器从内部环境和外部大气环境中采集生物气溶胶样本。基于Illumina MiSeq测序结果，对细颗粒和粗颗粒、舍内生物气溶胶样本、粪便样本以及舍内和舍外生物气溶胶样本中的抗生素抗性细菌进行生物学差异的比较分析。还研究了导致上述差异的关键属。结果表明，空气传播红霉素抗性细菌的生物多样性高于空气传播四环素抗性细菌，舍内生物气溶胶样本的生物多样性高于粪便样本。细颗粒和粗颗粒之间空气传播抗生素抗性细菌的生物多样性和群落结构没有显著差异。放线菌是导致红霉素抗性细菌与其他细菌种群之间差异的关键细菌之一。是与红霉素抗性和所有细菌菌群区分开来的四环素抗性菌群的关键属之一。群落结构结果表明，四环素和红霉素抗性细菌的优势菌群和群落结构没有显著差异。粪便和生物气溶胶样本在属水平上的群落结构不同，优势细菌也不同。本研究结果为准确评估动物养殖场抗生素抗性细菌的现状及其生态风险提供了基础数据。
