Arfken Ann M, Song Bongkeun, Sung Jung-Suk
Department of Biology and Marine Biology, University of North Carolina Wilmington, NC 28403, USA.
Department of Biological Sciences, Virginia Institute of Marine Sciences, Gloucester Point, VA, 23062, USA.
J Microbiol Biotechnol. 2015 May;25(5):709-17. doi: 10.4014/jmb.1408.08005.
Airborne bacteria from hog farms may have detrimental impacts on human health, particularly in terms of antibiotic resistance and pathogen zoonosis. Despite human health risks, very little is known about the composition and diversity of airborne bacteria from hog farms and hog-related spray fields. We used pyrosequencing analysis of 16S rRNA genes to compare airborne bacterial communities in a North Carolina hog farm and lagoon spray field. In addition, we isolated and identified antibiotic-resistant bacteria from both air samples. Based on 16S rRNA gene pyrosequence analysis, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the dominant phyla in airborne bacterial communities from both hog farm and spray field sites. Within the Firmicutes genera, Clostridium spp. were more abundant in the hog farm, whereas Staphylococcus spp. were higher in the spray field. The presence of opportunitic pathogens, including several Staphylococcus species and Propionibacterium acnes, was detected in both bioaerosol communities based on phylogenetic analysis. The isolation and identification of antibiotic-resistant bacteria from air samples also showed similar results with dominance of Actinobacteria and Proteobacteria in both hog farm and spray field air. Thus, the existence of opportunistic pathogens and antibiotic resistant bacteria in airborne communities evidences potential health risks to farmers and other residents from swine bioaerosol exposure.
来自养猪场的空气传播细菌可能会对人类健康产生不利影响，尤其是在抗生素耐药性和病原体人畜共患病方面。尽管存在人类健康风险，但对于来自养猪场和与猪相关的喷洒区域的空气传播细菌的组成和多样性，我们知之甚少。我们使用16S rRNA基因焦磷酸测序分析来比较北卡罗来纳州一个养猪场和泻湖喷洒区域的空气传播细菌群落。此外，我们还从空气样本中分离并鉴定了抗生素抗性细菌。基于16S rRNA基因焦磷酸测序分析，放线菌门、拟杆菌门、厚壁菌门和变形菌门是养猪场和喷洒区域空气传播细菌群落中的主要门类。在厚壁菌门的属中，梭菌属在养猪场更为丰富，而葡萄球菌属在喷洒区域含量更高。基于系统发育分析，在两个生物气溶胶群落中均检测到了包括几种葡萄球菌属和痤疮丙酸杆菌在内的机会性病原体。从空气样本中分离和鉴定抗生素抗性细菌也显示了类似的结果，放线菌门和变形菌门在养猪场和喷洒区域空气中均占主导地位。因此，空气传播群落中机会性病原体和抗生素抗性细菌的存在证明了猪生物气溶胶暴露对农民和其他居民存在潜在的健康风险。
