[游离羟胺对两种典型亚硝酸盐氧化细菌活性的影响]
[Effect of Free Hydroxylamine on the Activity of Two Typical Nitrite-oxidizing Bacteria].
作者信息
Shen Chen, Zhang Shu-Jun, Peng Yong-Zhen
机构信息
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Research and Development Center of Beijing Drainage Group Corporation, Beijing 100022, China.
出版信息
Huan Jing Ke Xue. 2020 Jun 8;41(6):2805-2811. doi: 10.13227/j.hjkx.201911110.
The sludge from enrichment of and was used as a research object and batch tests were performed. The inhibitory effects of hydroxylamine on and under the same pH and different hydroxylamine concentration gradients, the same hydroxylamine concentration, and different pH gradients were investigated. The results showed that under the same pH condition, the activity of decreased with increasing hydroxylamine concentration. Under the same hydroxylamine concentration (HA=5 mg·L) at a higher pH environment (pH ≥ 7.5), hydroxylamine produced more free hydroxylamine (FHA) and the inhibitory effect on was improved. At a low pH environment (pH≤7), ionic hydroxylamine promoted the activity of . The inhibitory effect of hydroxylamine on was limited. When pH=7.5 and hydroxylamine concentration was 45 mg·L, the relative activity of was 82%. The NOB growth rate kinetics model and the non-substrate inhibition linear equation were used to describe the effect of FHA on and activity. The coefficient of determination was 0.90 and 0.94, respectively. FHA may be the main reason for inhibiting the activity of and .
以亚硝酸盐氮（NO₂-N）和硝酸盐氮（NO₃-N）富集后的污泥为研究对象，进行了批次试验。研究了在相同pH值和不同羟胺浓度梯度、相同羟胺浓度和不同pH梯度下，羟胺对亚硝酸盐氧化菌（NOB）和硝酸盐氧化菌（NO₂B）的抑制作用。结果表明，在相同pH条件下，NOB的活性随羟胺浓度的增加而降低。在相同羟胺浓度（HA = 5 mg·L）下，在较高pH环境（pH≥7.5）中，羟胺产生更多的游离羟胺（FHA），对NOB的抑制作用增强。在低pH环境（pH≤7）中，离子态羟胺促进了NO₂B的活性。羟胺对NO₂B的抑制作用有限。当pH = 7.5且羟胺浓度为45 mg·L时，NOB的相对活性为82%。采用NOB生长速率动力学模型和非底物抑制线性方程来描述FHA对NOB和NO₂B活性的影响。决定系数R²分别为0.90和0.94。FHA可能是抑制NOB和NO₂B活性的主要原因。
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